A-172
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
A-172 |
Humane Glioblastom Zell-Linie |
300108 |
cryovial |
265,00 |
A-172 |
Humane Glioblastom Zell-Linie |
330108 |
vital |
335,00 |
| Designation: | A-172 |
| Depositor: | Giard |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 53 years |
| Gender: | male |
| Tissue: | brain |
| Celltype: | glioblastoma |
| Growth Properties: | monolayer |
| Description: | The A-172 cell line was established by D.J. Giard in 1973. |
| Culture Medium: | DMEM supplemented with 2 mM L-glutamine and 10% fetal bovine serum |
| Subculturing: | Remove medium and rinse with 0.02% EDTA solution. Add fresh 0.025% trypsin/0.02% EDTA solution and let the culture sit 37°C until the cells detach. Add fresh medium, centrifuge, resuspend in fresh medium and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:8 is recommended |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | no |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 9,12 D13S317: 11 D16S539: 12 D5S818: 11,12 D7S820: 11 THO1: 6,9.3 TPOX: 8,11 vWA: 20 D3S1358: 14,18 D21S11: 28,32.2 D18S51: 12,13 Penta E: 5,10 Penta D: 9,13 D8S1179: 13,14 FGA: 20,22 CLS ∙ Cell Lines Service, 2010. |
References: Giard DJ et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst 51: 1417-23, 1973. Pinkerton H and Rana MW. Cytotoxic effects of 1,3-bis(2 chloroethyl)-1-nitrosourea (BCNU) on cultured human glioblastomas. Proc Soc Exp Biol Med 151:532-4, 1976. Olopade OI et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res 52: 2523-9, 1992. Debinski W et al. Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J Biol Chem 271: 22428-33, 1996. Cordes N et al. beta1-integrin-mediated signaling essentially contributes to cell survival after radiation-induced genotoxic injury. Oncogene 25: 1378-90, 2006. Ohnishi K et al. Induction of radiation resistance by a heat shock protein inhibitor, KNK437, in human glioblastoma cells. Int J Radiat Biol 82: 569-75, 2006. Shirato K et al. Phenoxazine derivatives induce caspase-independent cell death in human glioblastoma cell lines, A-172 and U-251 MG. Oncol Rep 17: 201-8, 2007. | |
