CCF-STTG1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
CCF-STTG1 |
Humane Astrozytom Zell-Linie |
300388 |
cryovial |
265,00 |
CCF-STTG1 |
Humane Astrozytom Zell-Linie |
330388 |
vital |
335,00 |
| Designation: | CCF-STTG1 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 68 years |
| Gender: | Female |
| Tissue: | Brain |
| Morphology: | astrocytoma, grade IV |
| Celltype: | astrocytic |
| Culture Medium: | DMEM:Ham’s F12 media supplemented with 2 mM glutamine, 15 mM HEPES and 5% fetal bovine serum. |
| Subculturing: | Remove medium of sub-confluent cultures and rinse with 0.02% EDTA solution. Add fresh 0.025% trypsin / 0.02% EDTA solution and let the culture sit at 37°C until cells detach. Add fresh medium, centrifuge and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:8 is recommended |
| Fluid Renewal: | Every 2 to 3 days. |
| Freeze Medium: | CM-1 (Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Antigen Expression: | HLA DR (on approx. 25% of cells) |
References: In Vitro 19; 275,1983. Fed.Proc. 43: 781, 1984. Barna BP, Chou SM, Jacobs B, Ransohoff RM, Hahn JF, Bay JW. Enhanced DNA synthesis of human glial cell exposed to human leukocyte products. J Neuroimmunol 10(2): 151-158, 1985. Krul ES, Tank J. Secretion of apolipoprotein E by an astrocytoma cell line. J Neurosci Res 32(2): 227-238, 1992. Beaman L, Beaman BL. Differences in the interactions of Nocardia asteroides with macrophage, endothelial, and astrocytoma cell lines. Infect Immun 62(5): 1787-1798, 1994. Thilakawardhana S, Everett DM, Murdock PR, Dingwall C, Owen JS. Quantification of apolipoprotein E receptors in human brain-derived cell lines by real-time polymerase chain reaction. Neurobiol Aging 26(6): 813-823, 2005. | |
