GIMEN
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
GIMEN |
Humane Neuroblastom Zell-Linie |
300179 |
cryovial |
450,00 |
GIMEN |
Humane Neuroblastom Zell-Linie |
330179 |
vital |
495,00 |
| Designation: | GIMEN |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Tissue: | neuroblastoma |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | IFN gamma inhibits growth of GIMEN. FGF-2 is antimitogenic for GIMEN cells, this antimitogenic effect of FGF-2 is reversed by IFN gamma and enhanced by IL-1 beta. |
| Culture Medium: | Minimum essential medium Eagle supplemented with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C)until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:6 to 1:12 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
References: Cornaglia-Ferraris P, Ponzoni M, Montaldo P, Mariottini GL, Donti E, Di Martino D, Tonini GP. A new human highly tumorigenic neuroblastoma cell line with undetectable expression of N-myc. Pediatr Res. 1990 Jan;27(1):1-6. Scheil S, Ludecke G, Unsicker K. Interleukin-1 beta and interferon gamma interact with fibroblast growth factor-2 in the control of neuroblastoma cell proliferatin and differentiation. Int J Dev Neurosci 12(5): 405-10, 1994. Janet T, Ludecke G, Otten U, Unsicker K. Heterogeneity of human neuroblastoma cell lines in their proliferative responses to basic FGF, NGF, and EGF: correlation with expression of growth factors and growth factor receptors. J Neurosci Res. 40(6): 707-15, 1995. Carr J, Bell E, Pearson AD, Kees UR, Beris H, Lunec J, Tweddle DA. Increased frequency of aberrations in the p53/MDM2/p14(ARF) pathway in neuroblastoma cell lines established at relapse. Cancer Res. 2006 Feb 15;66(4):2138-45. | |
