H-4
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
H-4 |
Humane Neuronale Gliom Zell-Linie |
300184 |
cryovial |
265,00 |
H-4 |
Humane Neuronale Gliom Zell-Linie |
330184 |
vital |
335,00 |
| Designation: | H-4 |
| Depositor: | Riggs |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 37 years |
| Gender: | male |
| Tissue: | Brain |
| Morphology: | epithelial |
| Celltype: | Glioma |
| Growth Properties: | monolayer |
| Culture Medium: | Dulbecco's modified Eagle's medium supplemented with 2 mM L-glutamine, 4.5 g/L glucose and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin solution for 1 minute, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:10 to 1:15 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | no |
| Karyotype: | modal number = 75; range 45 = 80; Y chromosome present |
| Isoenzymes: | G6PD, B; PGM1, 1-2; PGM3, 1; ES-D, 1; Me-2, 0; AK-1, 1; GLO-1, 2; Phenotype Frequency Product: 0.0452 |
References: Arnstein, Taylor DO, Nelson-Rees WA, Huebner RJ, Lennette EH. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71-84, 1974. Day RS and Ziolkowski CH. Human brain tumour cell strains with deficient host-cell reactivation of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenovirus 5. Nature 279: 797-799, 1979. Krex D, Mohr B, Hauses M, Ehninger G, Schackert HK, Schackert G. Identification of uncommon chromosomal aberrations in the neuroglioma cell line H4 by spectral karyotyping. J Neurooncol. 52(2):119-28, 2001. | |
