HBL-52
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HBL-52 |
Humane Neuronale Meningiom Zell-Linie |
300188 |
cryovial |
375,00 |
HBL-52 |
Humane Neuronale Meningiom Zell-Linie |
330188 |
vital |
465,00 |
| Designation: | HBL-52 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Age/Stage: | 47 years old |
| Gender: | female |
| Tissue: | Brain |
| Morphology: | epithelial |
| Celltype: | Meningioma |
| Growth Properties: | monolayer |
| Description: | The cell line was originally taken from a transitional meningioma grade I localized at the optic canal. |
| Culture Medium: | DMEM:Ham's F12 (1:1 mixture) supplemented with 2 mM L-glutamine and 10% fetal bovine serum may be used as an alternative. |
| Subculturing: | Remove medium and rinse with fresh 0.02% EDTA solution. Rinse with 0.025% trypsin/0.02% EDTA solution, remove trypsin and let the culture sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 5 days. |
| Split Ratio: | A ratio of 1:2 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS - Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Tumorigenic: | yes, in nude mice |
References: Akat K., Mennel H.-D., Kremer P., Gassler N., Bleck C.K.E. and Kartenbeck J. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106: 337-347, 2003. | |
