HS-683
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HS-683 |
Humane Neuronale Gliom Zell-Linie |
300213 |
cryovial |
265,00 |
HS-683 |
Humane Neuronale Gliom Zell-Linie |
330213 |
vital |
335,00 |
| Designation: | Hs 683 |
| Depositor: | Owens |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 76 years |
| Gender: | male |
| Tissue: | brain |
| Morphology: | fibroblast |
| Celltype: | glioma |
| Growth Properties: | monolayer |
| Description: | Hs 683 cells were isolated from explant cultures of a glioma taken from the left temporal lobe of a 76 year old male Caucasian. Microvilli but no desmosomes were observed. |
| Culture Medium: | Dulbecco's modified Eagle's medium (4.5 g/L glucose) supplemented with 2 mM L-glutamine and 10% fetal bovine serum |
| Subculturing: | Remove medium and rinse with 0.02% EDTA solution. Add fresh 0.025% trypsin/0.02% EDTA solution and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS · Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | no |
| Karyotype: | (P15) hypotetraploid with mode = 88; range = 44 to 97; Y chromosomes present |
| Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 1-2; ES-D, 1; Me-2, 2; AK-1, 1; GLO-1, 2; Phenotype Frequency Product: 0.0029 |
References: Owens RB et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. Gershwin ME et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. Debinski W et al. Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. Pachiappan A et al. Glial inflammation and neurodegeneration induced by candoxin, a novel neurotoxin from Bungarus candidus venom: global gene expression analysis using microarray. Toxicon. 46(8):883-99, 2005. | |
