IMR-32
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
IMR-32 |
Humane Neuroblastom Zell-Linie |
300148 |
cryovial |
265,00 |
IMR-32 |
Humane Neuroblastom Zell-Linie |
330148 |
vital |
335,00 |
| Designation: | IMR-32 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 13 months |
| Gender: | male |
| Tissue: | brain (neuroblastoma) |
| Morphology: | fibroblast |
| Celltype: | Neuroblast, fibroblast |
| Growth Properties: | monolayer |
| Description: | There are two cell types present. A small neuroblast- like cell is predominant, and the other one is a large hyaline fibroblast. This cell line can be propagated to >80 serial subcultures. |
| Culture Medium: | Minimum essential medium Eagle supplemented with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate and 10% heat-inactivated fetal bovine serum. Alternatively, DMEM:Ham's F12 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum may be used. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin, 0.02% EDTA solution, rinse, remove trypsin, EDTA and allow the cultures to sit at room temperature (or 37°C for a short time period) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X/Y CSF1PO: 11/12 D13S317: 9/9 D16S539: 8/10 D5S818: 11/12 D7S820: 9/10 THO1: 7/9,3 TPOX: 11/11 vWA: 15/15 D3S1358: 16/16 D21S11: 30/31 D18S51: 12/15 Penta E: 7/15 Penta D: 11/12 D8S1179: 13/13 FGA: 21/24 CLS ∙ Cell Lines Service, 2009. |
| Isoenzymes: | G6PD, B |
| Reverse Transcriptase: | negative |
| Virus Resistance: | echovirus 11 |
| Virus Susceptibility: | vesicular stomatitis (Indiana); herpes simplex; vaccinia; coxsackievirus B3; poliovirus 3 (poorly) |
References: Tumilowicz JJ et al. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res 30: 2110-8, 1970. Maestrini E et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc Natl Acad Sci USA 93: 674-8, 1996. Rostomily RC et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res 57: 3526-31, 1997. Mangoura d et al. 12-O-tetradecanoyl-phorbol-13-acetate-dependent up-regulation of dopaminergic gene expression requires Ras and neurofibromin in human IMR-32 neuroblastoma. J Neurochem 97: 97-103, 2006. Malisauskas M et al. Intermediate amylid oligomers of lysozyme: Is their cytotoxicity a particular case or general rule for amyloid? Biochemistry (Mosc) 71: 505-12, 2006. Kaur M et al. A tuber lectin from Arisaema jacquemontii Blume with anti-insect and anti-proliferatve properties. J Biochem Mol Biol 39: 432-40, 2006. | |
