SH-SY5Y
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SH-SY5Y |
Humane Neuroblastom Zell-Linie |
300154 |
cryovial |
265,00 |
SH-SY5Y |
Humane Neuroblastom Zell-Linie |
330154 |
vital |
335,00 |
| Designation: | SH-SY5Y |
| Depositor: | Biedler |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 4 years old |
| Gender: | female |
| Tissue: | Brain (from metastatic site: bone marrow) |
| Morphology: | The cells grow as clusters of neuroblastic cells with multiple, short, fine cell processes (neurites). Cells will aggregate, form clumps and float; a confluent monolayer is not formed. |
| Celltype: | Neuroblast (neuroblastoma) |
| Growth Properties: | Monolayer; form clumps at high cell density |
| Description: | SH-SY5Y is one of three serially isolated neuroblast clones (SH-SY, SH-SY5, SH-SY5Y) of the human neuroblastoma cell line SK-N-SH which was established in 1970 from a metastatic bone tumor. The cells exhibit moderate levels of dopamine beta hydroxylase activity. They can convert glutamate to the neurotransmitter GABA. SH-SY5Y cells have a reported saturation density greater than 1 x 106 cells/cm2. The loss of neuronal characteristics has been described with increasing passage numbers (approx. passage 20). Neuronal markers or uptake of noradrenalin should be determined routinely. It is recommended not to use at passage numbers higher than 20 unless neuronal characteristics are controlled. |
| Passage: | 36 |
| Culture Medium: | DMEM:Ham's F12 (1:1 mixture) supplemented with 2 mM L-glutamine and 5-10% fetal bovine serum. |
| Subculturing: | These cells grow as a mixture of floating and adherent cells. Remove the medium with the floating cells, and recover the cells by centrifugation. Rinse the adherent cells with fresh 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution, and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 is recommended |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | Forms tumors in nude mice within approx. 3-4 weeks. |
References: Biedler JL et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res 38: 3751-7, 1978. Odelstad L et al. Neuron-specific enolase in relation to differentiation in human neuroblastoma. Brain Res 224: 69-82, 1981. Pahlman S et al. Phenotypic changes of human neuroblastoma cells in culture induced by 12-O-tetradecanoyl-phorbol-13-acetate. Int J Cancer 28: 583-9, 1981. Ross RA et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J Natl Cancer Inst 71: 741-7, 1983. Woo SY et al. Arsenic trioxide inhibits cell growth in sh-sy5y and sk-N-as neuroblastoma cell lines by a different mechanism. Pediatr Hematol Oncol 23: 231-43, 2006. Shavali S et al. Reactive macrophages increase oxidative stress and alpha-synuclein nitration during death of dopaminergic neuronal cells in co-culture:relevance to Parkinson`s disease. Neurochem Res 31: 85-94, 2006. Wang X et al. The proline-rich domain and the microtubule binding domain of protein tau actingas RNA binding domains. Protein Pept Lett 13: 679-85, 2006. | |
