SK-N-LO
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SK-N-LO |
Humane Neuroblastom Zell-Linie |
300400 |
cryovial |
375,00 |
SK-N-LO |
Humane Neuroblastom Zell-Linie |
330400 |
vital |
465,00 |
| Designation: | SK-N-LO |
| Depositor: | Buck |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Tissue: | Brain |
| Morphology: | epithelial |
| Celltype: | neuroblastoma |
| Growth Properties: | Adherent, on collagen-coated flasks |
| Culture Medium: | Minimum essential medium Eagle supplemented with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with fresh PBS. For detachment, use either 0.25% trypsin solution or the trypsin-alternatives Accutase or ScattEx (from CLS). Incubate the cells at 37°C until the cells detach. Add fresh medium, remove trypsin by centrifugation, add fresh medium and dispense into new flasks. Attachment of Sk-N-LO cells is enhanced on collagen-coated flasks. |
| Split Ratio: | A ratio of 1:6 to 1:12 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Karyotype: | Phenotype Frequency Product: 0.00005 |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,11 D16S539: 12 D5S818: 11,12 D7S820: 11 THO1: 10 TPOX: 8,11 vWA: 14,17 D3S1358: 14,17 D21S11: 27,28 D18S51: 12 Penta E: 7 Penta D: 9,13 D8S1179: 12,15 FGA: 25 CLS ∙ Cell Lines Service, 2010. |
References: Bruchelt G et al. [Effect of lithium on the proliferation of fibroblasts and tumor cell lines in vitro] Klin Padatr 197: 249-52, 1985. Buck J et al. Specific uptake of m-[125I]iodobenzylguanidine in the human neuroblastoma SK-N-SH. Cancer Res 45: 6366-70, 1985. Bruchelt G et al. Cytotxic effects of m-(125) iodobenzylguanidine on the human neuroblastoma cell lines SK-N-SH and SK-N-LO. Cancer Res 48: 2993-7, 1988. Baader SL et al. Uptake and cytotoxicity of ascorbic acid and dehydroascorbic acid in neuroblastoma (SK-N-LO) cells. Anticancer Res 14: 221-7, 1994. Lode HN et al. Ascorbic acid induces lipid peroxidation on neuroectodermal SK-N-LO cells with high endogenous ferritin content and loaded with MAb-ferritin immunoconjugates. Anticancer Res 14: 1903-6, 1994. Mayer P et al. Activation of cellular cytotoxicity and complement-mediated lysis of melanoma and neuroblastoma cells in vitro by murine antiganglioside antibodies MB 3.6 and 14.G2a. Melanoma Res 4: 101-6, 1994. Meyer T et al. Effects of cyclophosphamide and ifosfamide on neuroblastoma cells before and after activation by microsomes. Anticancer Res 17: 981-6, 1997. Heiss P et al. Cytotoxic effect of immunoconjugate composed of glucose-oxidase coupled to an anti-ganglioside (GD2) antibody on spheroids. Anticancer Res 17: 3177-8, 1997. Kuci Z et al. Bone marrow-derived factors support growth of N-type, but not of melanocytic neuroblastoma cells. Anticancer Res 22: 4325-9, 2002. Voigt A et al. Effects of retinoic acid on proliferation, apoptosis, cytotoxicity, migration, and invasion of neuroblastoma cells. Med Pediatr Oncol 40: 205-13, 2003. | |
