SK-N-MC
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SK-N-MC |
Humane Neuronale Epitheliom Zell-Linie |
300340 |
cryovial |
265,00 |
SK-N-MC |
Humane Neuronale Epitheliom Zell-Linie |
330340 |
vital |
335,00 |
| Designation: | SK-N-MC |
| Depositor: | Old |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 14 years |
| Gender: | female |
| Tissue: | Brain; from metastatic site: supra-orbital area |
| Morphology: | epithelial |
| Celltype: | Askin's tumor (ESFT= Ewing Sarcoma Family of Tumors) |
| Growth Properties: | monolayer |
| Description: | This cell line has been established by J.L. Biedler in 1971. It has moderate dopamine-beta-hydroxylase activity as well as formaldehyde induced fluorescence indicative of intracellular catecholamines. |
| Culture Medium: | DMEM:F12 (1:1 mixture) supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA (versene). Add fresh 0.025% trypsin/0.02% EDTA (versene) solution and let the culture sit at 37°C until the cells detach. Remove trypsin by centrifugation, add fresh medium and dispense into new flasks. |
| Split Ratio: | A ratio of 1:6 to 1:12 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice and also in hamster cheek |
| Karyotype: | hypodiploidy to pseudodiploidy. Abnormalities including double minutes, breaks; large submetacentric, telocentric and small telocentric markers (originator). (P32) Hypodiploid to hyperdiploid and triploid to hypotetraploid with abnormalities including dicentrics, breaks, double minutes (DM), large subtelocentric and small telocentric chromosomes. |
| Antigen Expression: | Blood Type O; Rh+ |
| Isoenzymes: | Me-2, 2; PGM3, 1-2; PGM1, 1; ES-D, 2; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.00005 |
References: Biedler JL et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res 33: 2643-52, 1973. Helson L et al. Human neuroblastoma in nude mice. Cancer Res 35: 2594-9, 1975. Biedler JL et al. Metaphase chromosome anomaly: association with drug resistance and cell-specific products. Science 191: 185-7, 1976. Biedler JL et al. A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture. J Natl Cancer Inst 57: 683-95, 1976. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumos in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Seeger RC et al. Morphology growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res 37: 1364-1371, 1977. Barnes EN et al. The fine structure of continuous human neuroblastoma lines SK-N-SH, SK-N-BE(2), and SK-N-MC. In Vitro 17: 619-31, 1981. Dunn T et al ERG gene is translocated in a Ewing's sarcoma cell line. Cancer Genet Cytogenet 76: 19-22, 1994. Rostomily RC et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res 57: 3526-3531, 1997. Morash SC et al. Expression of MARCKS effector domain mutants alters phospholipase D activity and cytoskeletal morphology of SK-N-MC neuroblastoma cells: Neurochem Res 30: 1353-1364, 2005. Demaegdt H et al. Angiotensin AT(4) receptor ligand interaction with cystinyl aminopeptidase and aminopeptidase N: [(125)I]Angiotensin IV only binds to the cystinyl aminopeptidase apo-enzyme. Eur J Pharmacol 546: 19-27, 2006. Zafar KS et al. Overexpression of NQO1 protects human SK-N-MC neuroblastoma cells against dopamine-induced cell death. Toxicol Lett 166: 261-7, 2006. | |
