T-406
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
T-406 |
Humane Hirn Glioblastom Zell-Linie |
300361 |
cryovial |
375,00 |
T-406 |
Humane Hirn Glioblastom Zell-Linie |
330361 |
vital |
465,00 |
| Designation: | T-406 |
| Depositor: | Lichtenthaler |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Tissue: | Brain |
| Morphology: | fibroblast |
| Celltype: | glioblastoma |
| Growth Properties: | monolayer |
| Culture Medium: | Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, add fresh 0.25% trypsin solution for 1 minute, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
References: Henn W et al. Polysomy of chromosome 7 is correlated with overexpression of the erbB oncogene in human glioblastoma cell line. Hum Genet 74: 104-6, 1986. Malter M et al. Natural cytotoxic cells from rat liver and spleen kill human glioma cells. J Cancer Res Clin Oncol 113: 498-501, 1987. Todorov DK et al. Antiproliferative activity of the non-myelotoxic antitumor agent of plant origin, Thaliblastine, on two human glioma cell lines. J Cancer Res Clin Oncol 118: 83-6, 1992. | |
