U-118 MG
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
U-118 MG |
Humane Neuronale Glioblastom Zell-Linie |
300362 |
cryovial |
265,00 |
U-118 MG |
Humane Neuronale Glioblastom Zell-Linie |
330362 |
vital |
335,00 |
| Designation: | U-118 MG |
| Depositor: | Ponten |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Age/Stage: | 50 years old |
| Gender: | male |
| Tissue: | Brain |
| Morphology: | mixed |
| Celltype: | glioblastoma (grade III) |
| Growth Properties: | monolayer |
| Description: | This is one of a number of cell lines derived from malignant gliomas (see also U-87 MG, U-138 MG and U-373 MG) by J. Ponten and associates from 1966 to 1969. |
| Culture Medium: | Dulbecco's modified Eagle's medium, 90%; fetal bovine serum, 10% - OR - Minimum essential medium (Eagle) in Earle's BSS with nonessential amino acids and sodium pyruvate, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at 37°C for about 3 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Karyotype: | The line has a near pentaploid chromosome number and a wide range of chromosome number distribution (40% of the cells had numbers ranging from 110 to 115). The following 14 markers were found in most metaphases: t(1p,2p), t(3p,?), t(4p,11q), t(7p,22q), M6, t(9q,?), i(11q)18q t(10q,?), M14, M15, M16, M17 and t(10q,22q); 6 of these were found in some and 10 were seen in one only. Normal chromosomes 7, 8, 12, 19, 20 and 22 had 5 to 6 copies per cell; the X had two copies and the Y was absent. |
| Antigen Expression: | Blood Type A, Rh+; HLA Aw24, A28, B12, Bw47 |
| Isoenzymes: | Me-2, 1; PGM3, 2; PGM1, 2; ES-D, 1; AK-1, 1-2; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0001 |
References: Ponten J et al. Long term culture of normal and neoplastic human glia. Acta Pathol Microbiol Scand 74: 465-86, 1968. Beckman G et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum Hered 21: 238-41, 1971. Vaheri A et al. A common cell-type specific surface antigen in cultured human glial cells and fibroblasts: loss in malignant cells. J Exp Med 143: 64-72, 1976. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Bluestein HG. Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus. Proc Natl Acad Sci USA 75: 3965-9, 1978. Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J Natl Cancer Inst 66: 1003-12, 1981. Olopade Oi et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-9, 1992. Cairns P et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res 57: 5356-9, 1997. Strakova N et al. Peroxisome proliferator-activated receptors (PPAR) agonists affect cell viability, apoptosis and expression of cell cycle related proteins in cell lines of glial brain tumors. Neoplasma 52: 126-36, 2005. Gross D et al. Platelet-derived growth factor receptor independent proliferation of human glioblastoma cells: selective tyrosine kinase inhibitors lack antiproliferative activity. J Cancer Res Clin Oncol 132: 589-99, 2006. | |
