U-138 MG
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
U-138 MG |
Humane Neuronale Glioblastom Zell-Linie |
300363 |
cryovial |
265,00 |
U-138 MG |
Humane Neuronale Glioblastom Zell-Linie |
330363 |
vital |
335,00 |
| Designation: | U-138 MG |
| Depositor: | Ponten |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Age/Stage: | 47 years |
| Gender: | male |
| Tissue: | Brain |
| Morphology: | polygonal |
| Celltype: | glioblastoma |
| Growth Properties: | monolayer |
| Description: | This is one of a number of cell lines derived from malignant gliomas, e.g. U-87-MG, U-118-MG and U-373-MG isolated by J. Ponten and associates from 1966 to 1969. It differs from U-87-MG in morphology and it has a slower proliferation rate. U-138-MG shows strong similarity to U-118-MG, sharing at least six derivative marker chromosomes |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS supplemented with 2 mM L-glutamine, 1% nonessential amino acids, 1 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin solution for 1 minute, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:4 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | no |
| Karyotype: | Hyperdiploid to pentaploid with several markers; the stemline chromosome number is near triploid with the 2S component occurring at 9.8%. Five markers [t(11;5), t(8q;4), t(19;?18), M1 and M2] were common to most S metaphases. One chromosome 4 could be found in every S metaphase. Chromosome composition was very uniform among cells. |
| DNA Profile (STR): | Amelogenin: X/Y CSF1PO: 12 D13S317: 9/11 D16S539: 12/13 D5S818: 11 D7S820: 9 THO1: 6 TPOX: 8 vWA: 18 D3S1358: 15 D21S11: 27/32.2 D18S51: 13 Penta E: 7 Penta D: 9/13 D8S1179: 14/15 FGA: 18/23 CLS ∙ Cell Lines Service, 2009. |
| Antigen Expression: | Blood Type A; Rh+ |
| Isoenzymes: | Me-2, 1; PGM1, 1; PGM3, 1; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0511 |
References: Ponten J et al. Long term culture of normal and neoplastic human glia. Acta Pathol Microbiol Scand 74: 465-86, 1968. Beckman G et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum Hered 21: 238-41, 1971. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-214, 1977. Werner-Felmayer G et al. Characteristics of interferon induced tryptophan metabolism in human cells in vitro. Biochim Biophys Acta 1012: 140-7, 1989. Olopade OI et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res 52: 2523-9, 1992. Koochekpour S et al. Met and hepatocyte growth factor/scatterc factor expression in human gliomas. Cancer Res 57: 5391-8, 1997. Cairns P et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res 57: 5356-9, 1997. Hu X et al. Relative gene-silencing efficiencies od small interfering RNAs targeting sense and antisense transcripts from the same genetic locus. Nucleic Acids Res 32: 4609-17, 2004. Mahe C et al. Functional expression of the serotonin 5-HT7 receptors in human glioblastoma cell lines. Br J Pharmalcol 143: 404-10, 2004. | |
