U-343 MGa
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
U-343 MGa |
Humane Neuronale Glioblastom Zell-Linie |
300365 |
cryovial |
375,00 |
U-343 MGa |
Humane Neuronale Glioblastom Zell-Linie |
330365 |
vital |
465,00 |
| Designation: | U-343 MGa |
| Depositor: | Senner |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Gender: | Male |
| Tissue: | Brain |
| Morphology: | epithelial |
| Celltype: | glioblastoma |
| Growth Properties: | monolayer |
| Description: | Comparison of STR-data has revealed, that the STR-profile of U-343 MGa cells stored at CLS is identical to GOS-3 described by DSMZ, Braunschweig. We aim to clarify this problem as soon as possible. |
| Culture Medium: | Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 5 days. When using Accutase for detachment, the cells will come off the substrate within 10min at 37°C. |
| Split Ratio: | A ratio of 1:2 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 9,13 D16S539: 9,12 D5S818: 12,13 D7S820: 9,11 THO1: 6,9.3 TPOX: 8,9 vWA: 17,18 D3S1358: 15,17 D21S11: 31,33.2 D18S51: 23 Penta E: 10,12 Penta D: 9,10 D8S1179: 13,14 FGA: 19,20 CLS ∙ Cell Lines Service, 2009. |
| Antigen Expression: | GFAP positive |
References: Vaheri A et al. A common cell-type specific surface antigen in cultured human glial cells and fibroblasts: loss in malignant cells. J Exp Med 143: 64-72, 1976. Ponten J et al. Properties of human malignant glioma cells in vitro. Med Biol 56: 184-93, 1978. Nister M et al. Clonal variation in the production of a platelet-derived growth factor-like protein and expression of corresponding receptors in a human malignant glioma. Cancer Res 46: 332-40, 1986. Stenerlow B et al. Clonogenic cell survival and rejoining of DNA double-strand breaks: comparisons between three cell lines after photon or He ion irradiation. Int J Radiat Biol 65: 631-9, 1994. Carlsson J et al. Cell type dependent effectiveness of tumour cell inactivation by radiation with increased ionisation density. Anticancer Res 15: 273-82, 1995. Enblad P et al. Modulation of phenotype and induction of irregular vessels accompany high tumorigenic potential of clonal human glioma cells xenografted to nude-rat barin. Int J Cancer 85: 819-28, 2000. Maletinska L et al. Human glioblastoma cell lines: levels of low-density lipoprotein receptor and low-density lipoprotein receptor-related protein. Cancer Res 60: 2300-3, 2000. | |
