U-87 MG
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
U-87 MG |
Humane Neuronale Glioblastom (Astrozytom) Zell-Linie |
300367 |
cryovial |
265,00 |
U-87 MG |
Humane Neuronale Glioblastom (Astrozytom) Zell-Linie |
330367 |
vital |
335,00 |
| Designation: | U-87 MG |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 44 years old |
| Gender: | female |
| Tissue: | Brain |
| Morphology: | epithelial |
| Celltype: | glioblastoma (grade IV) |
| Growth Properties: | monolayer |
| Description: | This is one of a number of cell lines derived from malignant gliomas which have been isolated by J. Ponten and associates from 1966 to 1969. |
| Culture Medium: | EMEM (EBSS) supplemented with 2 mM L-glutamine, 0.1 mM non-essential amino acids (NEAA), 1.0 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02 EDTA (versene) solution. Add freshly prepared 0.025% trypsin/0.02% EDTA solution and let the culture sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice inoculated subcutaneously with 107 cells. |
| Karyotype: | The stemline chromosome number is hypodiploid, with the modal chromosome number of 44 occurring in 48% of the cells. The rate of higher ploidity was 5.9%. The following twelve markers were common to all cells, including der(1)t(1;3) (p22;q21) der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. All S metaphases were monosomic for the X chromosome. Neither homogeneously staining regions (HSR) nor double minutes (DM) were detected, as was the case with N1, N6 and N9. |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 10,11 D13S317: 8,11 D16S539: 12 D5S818: 11,12 D7S820: 8,9 THO1: 9.3 TPOX: 8 vWA: 15,17 D3S1358: 16,17 D21S11: 28,32.2 D18S51: 13 Penta E: 7,14 Penta D: 9,14 D8S1179: 10,11 FGA: 18,24 CLS ∙ Cell Lines Service, 2010. |
| Antigen Expression: | Blood Type A, Rh+ |
| Isoenzymes: | Me-2, 1; PGM3, 1; PGM1, 2; ES-D, 1; AK-1, 1; GLO-1, 1; G6PD, B; Phenotype Frequency Product: 0.0017 |
References: Ponten J et al. Long term culture of normal and neoplastic human glia. Acta Path Microbiol Scand 74: 465-86, 1968. Beckman G et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum Hered 21: 238-41, 1971. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Starosta-Rubinstein, S. Imaging of a glioma using peripheral benzodiazepine receptor ligands. Proc. Natl. Acad. Sci. USA Vol. 84: 891-895,1987. Olopade OI et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res 52: 2523-9, 1992. Li YM et al. Molecular identity and cellular distribution of advanced glycation end product receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc Natl Acad Sci USA 93: 11047-52, 1996. Ozawa T et al. Response of intracerebral human glioblastoma xenografts to multifraction radiation exposures. Int J Radiat Oncol Biol Phys 66: 263-70, 2006. Dreses-Werringloer u et al. Chlamydophila (Clamydia) pneumoniae infection of human astrocytes and microglia in culture displays an active, rather than a persistent, phenotype. Am J Med Sci 332: 168-74, 2006. | |
