Chang-Liver
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
Chang-Liver |
Humane Leber Zell-Linie |
300139 |
cryovial |
315,00 |
Chang-Liver |
Humane Leber Zell-Linie |
330139 |
vital |
395,00 |
| Designation: | Chang Liver |
| Depositor: | Chang |
| Organism: | Homo sapiens (human) |
| Tissue: | Liver, normal |
| Morphology: | epithelial |
| Growth Properties: | monolayer, cells pile up at high density |
| Description: | Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. The cells are positive for keratin by immunoperoxidase staining. |
| Culture Medium: | Minimum Essential medium Eagle supplemented with 2 mM L-glutamine, 1% NEAA, 1 mM sodium pyruvate, and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with fresh 0.02% EDTA solution. Add fresh 0.025% trypsin/0.02% EDTA solution. Allow flask to sit at 37°C until cells detach. Add fresh medium, remove trypsin by centrifugation, add fresh medium and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS · Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in Syrian hamsters |
| HeLa Markers: | Yes |
| Isoenzymes: | G6PD, A |
| Reverse Transcriptase: | negative |
| Viruses: | Tested MHV (mouse hepatitis virus) negative |
| Virus Susceptibility: | poliovirus 1, 2, 3; adenovirus 3; vesicular stomatitis (Indiana) |
| Products: | keratin |
References: Chang RS. Continuous subcultivation of epithelial-like cells from normal human tissues. Proc. Soc. Exp. Biol. Med. 87: 440, 1954. Murphy WH, Landau BJ. Clonal variation and interaction of cells with viruses. Natl. Cancer Inst. Monogr. 7: 249, 1962. Benn J, Su F, Doria M, Schneider RJ. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. Chae S et al. Centrosome amplification and multirnuclear phenotypes are induced by hydrogen peroxide. Experimental and Molecular Medicine 37(5): 482-487, 2005. | |
