PLC-PRF-5
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
PLC-PRF-5 |
Humane Hepatom Zell-Linie |
300315 |
cryovial |
265,00 |
PLC-PRF-5 |
Humane Hepatom Zell-Linie |
330315 |
vital |
335,00 |
| Designation: | PLC-PRF-5 |
| Depositor: | Crawford |
| Organism: | Homo sapiens (human) |
| Tissue: | Liver |
| Morphology: | Epithelial |
| Celltype: | hepatoma; Alexander cells |
| Growth Properties: | monolayer |
| Description: | The cells produce HBsAg. At present, there is no evidence that this cell line produces infectious Hepatitis B virus. |
| Culture Medium: | DMEM medium supplemented with 2 mM Glutamine and 10% foetal bovine serum. |
| Subculturing: | Remove medium from sub-confluent cultures, add fresh 0.25% trypsin for 2 to 3 minutes, remove trypsin and let the culture sit at 37°C until the cells detach. Add fresh medium and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 is recommended; seeding density 2-3x104 cells/cm2. |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Biosafety Level: | 2 |
| Oncogene: | c-abl, c-fes, c-fms, c-myc, c-ha-ras, c-sis. |
| DNA Profile (STR): | Amelogenin: X / X CSF1PO: 10/10 D13S317: 11/12 D16S539: 13/13 D5S818: 12/12 D7S820: 9/9 THO1: 7/8 TPOX: 8/8 vWA: 15/16 D3S1358: 15/15 D21S11: 30/33.2 D18S51: 17/17 Penta E: 10/16 Penta D: 6/10 D8S1179: 13/16 FGA: 25/- CLS ∙ Cell Lines Service, 2009. |
| Products: | hepatitis virus B surface antigen (HBsAg) |
References: Alexander JJ et al. Establishment of a continuously growing cell line from primary carcinoma of the liver. S Afr Med J 50: 2124-8, 1976. MacNab GM et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Br J Cancer 34: 509-15, 1976. Monjardino J et al. Polypeptide profile of HBSAg excreted by a human hepatoma cell line. Virology 96: 652-5, 1979. Skelly J et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Nature 282: 617-8, 1979. Gilligan A et al. Variation in EGF-induced EGF receptor downregulation in human hepatoma- derived cell lines expressing different amounts of EGF receptor. Exp Cell Res 200: 235-41, 1992. Nakamoto N et al. Cyclooxygenase-2 inhibitor and interferon-beta synergistically induce apoptosis in human hepatoma cells in vitro and in vivo. Int J Oncol 29: 625-35, 2006. Koike K et al. Combination of 5-FU and IFNalpha enhances IFN signalling pathway and caspase-8 activity, resulting in marked apoptosis in hepatoma cell lines. Int J Oncol 29: 1253-61, 2006. | |
