SK-HEP-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SK-HEP-1 |
Humane Leber Adenokarzinom Zell-Linie |
300334 |
cryovial |
265,00 |
SK-HEP-1 |
Humane Leber Adenokarzinom Zell-Linie |
330334 |
vital |
335,00 |
| Designation: | SK-HEP-1 |
| Depositor: | Turner |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 52 years |
| Gender: | Male |
| Tissue: | Liver (ascites) |
| Morphology: | Endothelial (Heffelfinger, 1992) |
| Celltype: | Adenocarcinoma |
| Growth Properties: | Monolayer |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS with nonessential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C)until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | Yes, in nude mice; forms large cell carcinoma consistent with hepatoma |
| Karyotype: | (P11) hyperdiploid to hypotriploid (+A3, +C, +E, +F, +G, -A, -D) with abnormalities including dicentrics, acrocentric fragments, secondary constrictions, pulverizations, and large subtelocentric and submetacentric markers |
| Isoenzymes: | Me-2, 1-2; PGM3, 1; PGM1, 2; ES-D, 1; AK-1, 1; GLO-1, 1; G6PD, B; Phenotype Frequency Product: 0.0020 |
References: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Fogh J. Cultivation, characterization, and identification of human tumor cells with empasis on kidney, testis, and bladder tumors. Natl Cancer Inst Monogr 49: 5-9, 1978. Turner BM et al. Secretion of alpha1-antitrypsin by an established human hepatoma cell line and by human/mouse hybrids. Somatic Cell Genet 6: 1-14, 1980. Heffelfinger SC et al. SK HEP-1: a human cell line of endothelial origin. In Vitro Cell Dev Biol 28A: 136-142, 1992. Rebelo Sl et al. Modeling of tumor necrosis factor receptor superfamily 1A mutants associated with tumor necrosis factor receptor-associated periodic syndrome indicates misfolding consistent with abnormal function. Arthritis Rheum 54: 2674-87, 2006. Chen Ch et al. Decreased expressions of hepsin in human hepatocellular carcinomas. Liver Int 26: 774-80, 2006. | |
