HEP-G2
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HEP-G2 |
Humane Hepatom Zell-Linie |
300198 |
cryovial |
265,00 |
HEP-G2 |
Humane Hepatom Zell-Linie |
330198 |
vital |
335,00 |
| Designation: | HEP-G2 |
| Depositor: | W-Institute |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 15 years of age |
| Gender: | male |
| Tissue: | liver |
| Morphology: | epithelial |
| Celltype: | Hepatoblastoma (hepatocellular carcinoma) |
| Growth Properties: | monolayer |
| Description: | There is no evidence of a Hepatitis B virus genome in this cell line. The cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities; they demonstrate decreased expression of apoA-I mRNA and increased expression of catalase mRNA in response to gramoxone (oxidative stress). |
| Culture Medium: | DMEM:Ham’s F12 supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with fresh 0.02% EDTA solution. Add fresh 0.25% trypsin/0.02% EDTA solution and allow the flask to sit at 37°C until the cells detach. Add fresh medium, remove trypsin by centrifugation and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 to 1:6 is recommended |
| Fluid Renewal: | 2 times weekly |
| Doubling Time: | Approx. 48 hrs |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | no |
| Karyotype: | modal number = 55 (range = 50 to 60); has a rearranged chromosome 1 |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 9,13 D16S539: 12,13 D5S818: 11,13 D7S820: 10 THO1: 9 TPOX: 8,9 vWA: 17 D3S1358: 15,16 D21S11: 29,31 D18S51: 13,14 Penta E: 15,20 Penta D: 9,13 D8S1179: 15,16/17 FGA: 22,25 CLS ∙ Cell Lines Service, 2010. |
| Receptors Expressed: | insulin; insulin-like growth factor II (IGF II) |
| Products: | Albumin; alpha-fetoprotein (alpha fetoprotein); alpha1 acid glycoprotein (alpha-1 acid glycoprotein); alpha1 antitrypsin (alpha-1-antitrypsin); alpha1 antichymotrypsin; (alpha-1-antichymotrypsin); alpha2 HS glycoprotein (alpha-2-HS- glycoprotein); alpha2 macroglobulin (alpha-2-macroglobulin); beta lipoprotein (beta-lipoprotein); ceruloplasmin; C4 and C3 activator; fibrinogen; haptoglobin; plasminogen; retinol binding protein (retinol-binding protein); transferrin |
References: Aden DP et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. Knowles BB, Howe CC. Aden DP Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. Knowles BB and Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefore. U.S. Pat. 4,393,133. Diamond L, Rkuszewski F, Aden DP, Knowles BB, Baird WM. Metabolic activation of benzo(a)pyrene by a human hepatoma cell line. Carcinogenesis 1(10): 871-5, 1980. Zannis VI, Breslow JL, SanGiacomo TR, Aden DP, Knowles BB. Characterization of the major apolipoproteins secreted by two human hepatoma cell lines. Biochemistry. 20 (25):7089-96, 1981. Saito H, Goodnough LT, Knowles BB, Aden DP. Synthesis and secretion of alpha 2-plasmin inhibitor by established human liver cell lines. Proc Natl Acad Sci USA 79(18):5684-7, 1982. Simon D, Aden DP, Knowles BB. Chromosomes of human hepatoma cell lines. Int J Cancer 30(1):27-33, 1982. Moriarity DM, Fox N, Aden DP, Hoyer JR, Knowles BB. Identification of human hepatoma-defined cell surface molecules. Hybridoma 2(1):39-47, 1983. Moses AC, Freinkel AJ, Knowles BB, Aden DP. Demonstration that a human hepatoma cell line produces a specific insulin-like growth factor carrier protein. J Clin Endocrinol Metab 56(5):1003-8, 1983. Murata Y, Sarne DH, Horwitz AL, Lecocq R, Aden DP, Knowles BB, Refetoff S. Characterization of thyroxine-binding globulin secreted by a human hepatoma cell line. J Clin Endocrinol Metab 60(3):472-8, 1985. Darlington GJ et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. Busch SJ et al. Differential regulation of hepatic triglyceride lipase and 3-hydroxy-3- methylglutaryl-CoA reductase geneexpression in a human hepatoma cell line, HepG2. J. Biol. Chem. 265: 22474-22479, 1990. Benn J et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. Goodrum FD et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. Kolanus W et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. Lewis W et al. Fialuridine and its metabolites inhibit DNA polymerase gamma at sites of multiple adjacent analog incorporation, decrease mtDNA abundance, and cause mitochondrial structural defects in cultured hepatoblasts. Proc. Natl. Acad. Sci. USA 93: 3592-3597, 1996. Jang SI et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. Roesler WJ et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. Lee JH et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. Lieber A et al. Recombinant adenoviruses with large deletions geneerated by cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo. J. Virol. 70: 8944-8960, 1996. Dubuisson J and Rice CM. Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin. J.Virol. 70: 778-786, 1996. Yamaguchi Y et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. Kounas MZ et al. Cellular internalization and degradation of antithrombin III-thrombin, heparin cofactor II-thrombin, and alpha1-antitrypsin-trypsin complexes is mediated by the low density lipoprotein receptor-related protein. J. Biol. Chem. 271: 6523-6529, 1996. Klemm DJ et al. Adenovirus E1A proteins regulate phosphoenolpyruvate carboxykinase gene transcription through multiple mechanisms. J. Biol. Chem. 271: 8082-8088, 1996. Wu X et al. Demonstration of a physical interaction between microsomal triglyceride transfer protein and apolipoprotein B during the assembly of ApoB-containing lipoproteins. J. Biol. Chem. 271: 10277-10281, 1996. Ostlund RE J et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. Cuthbert C et al. Regulation of human apolipoprotein A-I gene expression by gramoxone. J. Biol. Chem. 272: 14954-14960, 1997. Deleersnyder V et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. | |
