HT-1080
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HT-1080 |
Humane Fibrosarkom Zell-Linie |
300216 |
cryovial |
265,00 |
HT-1080 |
Humane Fibrosarkom Zell-Linie |
330216 |
vital |
335,00 |
| Designation: | HT-1080 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 35 years old |
| Gender: | male |
| Tissue: | fibrosarcoma |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | The cells contain an activated N-ras oncogene. |
| Culture Medium: | DMEM:F12 (1:1 mixture) supplemented with 2 mM L- glutamine, 15 mM Hepes and 10% fetal bovine serum. |
| Subculturing: | Remove culture medium and rinse with 0.02% EDTA solution. Add fresh 0.025% trypsin, 0.02% EDTA solution and remove. Allow flask to sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS · Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in immunosuppressed mice |
| Oncogene: | ras+ |
| Karyotype: | Modal number: 2n=46, pseudodiploid |
| DNA Profile (STR): | Amelogenin: X ,Y CSF1PO: 12 D13S317: 12,14 D16S539: 9,12 D5S818: 11,13 D7S820: 9,10 THO1: 6 TPOX: 8 vWA: 14,19 D3S1358: 16 D21S11: 28,30 D18S51: 12,18 Penta E: 5,15 Penta D: 9,12 D8S1179: 13,14 FGA: 22,25 CLS ∙ Cell Lines Service, 2011. |
| Isoenzymes: | G6PD, B |
| Reverse Transcriptase: | negative |
| Virus Susceptibility: | poliovirus 1; vesicular stomatitis (Indiana); RD114; feline leukemia virus (FeLV) |
References: Rasheed S et al. Characterization of a newly derived human sarcoma cell line (HT-1080). Cancer 33: 1027-1033, 1974. Chen TR et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351, 1983. Slovak ML et al. Cytogenetic alterations associated with the acquisition of Doxorubicin resistance: Possible significance of chromosome 7 alterations. Cancer Research 47: 6646-6652, 1987. Geiser AG et al. Suppression of tumorigenicity in human cell hybrids derived from cell lines expressing different activated ras oncogenes. Cancer Res. 49: 1572-1577, 1989. Iida A et al. Inducible gene expression by retrovirus-mediated transfer of a modified tetracycline-regulated system. J. Virol. 70: 6054-6059, 1996. Brenneman M et al. Stimulation of intrachromosomal homologous recombination in human cells by electroporation with site-specific endonucleases. Proc. Natl. Acad. Sci. USA 93: 3608-3612, 1996. Hocking AM et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. Zaman MH et al. Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matirx adhesion and proteolysis. Proc Natl Acad Sci USA 103(29): 10889-10894, 2006. | |
