SW-684
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SW-684 |
Humane Fibrosarkom Zell-Linie |
300422 |
cryovial |
265,00 |
SW-684 |
Humane Fibrosarkom Zell-Linie |
330422 |
vital |
335,00 |
| Designation: | SW-684 |
| Depositor: | Leibovitz |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 68 years |
| Gender: | male |
| Tissue: | Connective tissue |
| Morphology: | fibroblast |
| Celltype: | fibrosarcoma |
| Growth Properties: | monolayer |
| Description: | The SW 684 cell line was initiated by A. Leibovitz in 1974 at the Scott and White Clinic, Temple, Texas from a fibrosarcoma removed from a 68 year old male Caucasian. |
| Culture Medium: | Leibovitz's L-15 medium supplemented with 5% fetal bovine serum |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin - 0.02% EDTA solution and let the culture sit at room temperature for two minutes. Remove trypsin, and let the culture sit at 37°C for 5 minutes. Add fresh medium to disperse the cells, and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, produces tumors in nude mice consistent with fibrosarcoma |
| Karyotype: | hypertriploid; modal number = 73; range = 59 to 79. The rate of higher ploidies was 9.1%. Eleven markers were common to most cells. These include: der(2)t(2;6)(p13;q13), der(12)t(8;12)(q11;q24), t(15q21q), 19q+, t(8p21q?) and six others. Of these, the der(2) and t(8p21q?) were generally paired. A few cells had double minutes (DM)(one per cell when present).There were 4 copies of N1, N18, N20 and N22 in most cells. Normal 15 and Y were absent. The X was paired in all cells. |
| Isoenzymes: | G6PD, B; PGM1, 1-2; PGM3, 1; AK-1, 1-2; GLO-1, 2; Phenotype Frequency Product: 0.0055 |
References: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. KishnaniNS et al. Identification and characterization of human tissue inhibitor of metalloproteinase-3 and detection of three additional metalloproteinase inhibitor activities in extracellular matrix. Matrix Biol 14: 479-88, 1995. Rodriques M et al. Comparative 99mTc-MIBI, 99mTc-furifosmin uptake in human soft tissue sarcoma cell lines. Eur J Nucl Med 27: 1839-43, 2000. Ding I et al. Comparison and modulation of angiogenic responses by FGFs, VEGF and SCF in murine and human fibrosarcomas. Comp Biochem Physiol A Mol Integr Physiol 132: 17-25, 2002. | |
