SW-872
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SW-872 |
Humane Liposarkom Zell-Linie |
300405 |
cryovial |
265,00 |
SW-872 |
Humane Liposarkom Zell-Linie |
330405 |
vital |
335,00 |
| Designation: | SW 872 |
| Depositor: | Leibovitz |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 36 years |
| Gender: | male |
| Tissue: | liposarcoma |
| Morphology: | fibroblast |
| Growth Properties: | monolayer |
| Description: | The SW 872 cell line was initiated by A. Leibovitz in 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a fibrosarcoma removed from a 36 year old male Caucasian. The histopathology evaluation reported an undifferentiated malignant tumor consistent with liposarcoma. |
| Culture Medium: | Leibovitz's L-15 medium supplemented with 2 mM L-glutamine and 5% fetal bovine serum. Alternatively, the cells may be cultured in DMEM, 4.5 g/L glucose, supplemented with 2 mM L-glutamine and 5% fetal bovine serum. (37°C, 5% CO2) |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin, 0.02% EDTA solution and let the culture sit at room temperature for two minutes. Remove trypsin, and let the culture sit at 37°C for 5 minutes. Add fresh medium to disperse the cells, and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, produces spindle cell sarcoma in nude mice consistent with liposarcoma |
| Karyotype: | hypertriploid; modal number = 80; range = 66 to 81. The rate of higher ploidies was 8.2%. Ten markers were common to most cells. These were: der(5)t(5;?)(q31;?)1, der(5)t(5;?)(q31;?)2, der(6)t(6;?)(q15;?), der(7)t(7;?)(q36;?), t(15q16q) and five others. Both der(5) markers, the der(7) and t(15q16q) were paired. There were 5 copies of N20 and N21, 4 copies of N8, N9, N11, N14 and N17 and a single copy of X in each cell. |
| Antigen Expression: | Blood type O+ |
| Isoenzymes: | G6PD, B; PGM1, 1-2; PGM3, 1; ES-D, 1; AK-1, 1; GLO-1, 1-2; Phenotype Frequency Product: 0.0708 |
References: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One-hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Hu M et al. Purification and characterization of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res 57: 3577-84, 1997. Achilles EG et al. Heterogeneity of angiogenic activity in a human liposarcoma: a proposed mechanism for “no take” of human tumors in mice. J Natl Cancer Inst 93: 1075-81, 2001. Cheema SK et al. Lack of stimulation of cholesteryl ester transfer protein by cholesterol in the presence of a high-fat diet. J Lipid Res 46: 2356-66, 2005. Chow WA et al. Nelfinavir induces liposarcoma apoptosis and cell cycle arrest by upregulating sterol regulatory element binding protein-1. Anticancer Drugs 17: 891-903, 2006. | |
