SW-982
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SW-982 |
Humane synoviale Axilla Zell-Linie |
300404 |
cryovial |
265,00 |
SW-982 |
Humane synoviale Axilla Zell-Linie |
330404 |
vital |
335,00 |
| Designation: | SW 982 |
| Depositor: | Leibovitz |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 25 years of age |
| Gender: | female |
| Tissue: | Synovium; synovial sarcoma; axilla |
| Morphology: | mixed |
| Growth Properties: | monolayer |
| Description: | The SW-982 cell line was initiated by A. Leibovitz in 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a biphasic synovial sarcoma removed from a 25 year old female Caucasian. The histopathology evaluation reported an undifferentiated malignant tumor consistent with liposarcoma. |
| Culture Medium: | DMEM supplemented with 2 mM L-glutamine and 5% fetal bovine serum. (37°C, 5-10% CO2) |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin - 0.02% EDTA solution and let the culture sit at room temperature for two minutes. Remove trypsin, and let the culture sit at 37°C for 5 minutes. Add fresh medium to disperse the cells, and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Karyotype: | hyperdiploid; modal number = 48; range = 42 to 58. The rate of higher ploidies was 1.6%.Nine markers were common to all cells. These were: t(1q4q), del(5)(q31;q33), der(9)t(4;9)(q11;p24), t(8q12p), t(9q13q) and four others. Double minutes (DM) were seen in some cells (usually only one copy). Normal N9 was absent, N4, N8, and N13 were consistently single-copied and the X was double-copied. |
| Isoenzymes: | G6PD, B; PGM1, 1-2; PGM3, 1-2; ES-D, 1; AK-1, 1; GLO-1, 1; Phenotype Frequency Product: 0.0192 |
References: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Tsuji F et al. Effects of mitogen-activated protein kinase inhibitors or phosphodiesterase inhibitors on interleukin-1-induced cytokines production in synovium-derived cells. Immunol Lett 68: 275-9, 1999. Rodrigues M et al. Comparative 99mTc-MIBI, 99mTc-Tetrofosmin and 99mTc-furifosmin uptake in human soft tissue sarcoma cell lines. Eur J Nucl Med 27: 1839-43, 2000. Yamazaki T et al. Phenotypic characterization of a human synovial sarcoma cell line, SW982, and its response to dexamethasone. In vitro Cell Dev Biol Anim 39: 337-9, 2003. Kochukov MY et al. Thermosensitive TRP ion channels mediate cytosolic calcium response in human synoviocytes. Am J Physiol Cell Physiol 291: C424-32, 2006. Joyner DE et al. Deoxyrubicin induces cell senescence preferentially over apoptosis in the FU-SY-1 synovial sarcoma cell line. J Orthop Res 24: 1163-9, 2006. | |
