ECV-304
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
ECV-304 |
Humane Harnblasen Karzinom Zell-Linie |
300452 |
cryovial |
265,00 |
ECV-304 |
Humane Harnblasen Karzinom Zell-Linie |
330452 |
vital |
335,00 |
| Designation: | ECV-304 |
| Depositor: | Takahashi |
| Organism: | Homo sapiens (human) |
| Synonym(s): | Derivative of T24 |
| Tissue: | urinary bladder; carcinoma |
| Morphology: | epithelial |
| Growth Properties: | adherent |
| Description: | DNA profiling studies, performed by ATCC, revealed that STR patterns of the endothelial line ECV-304 and the human bladder line T24 were very similar, suggesting that ECV-304 was a derivative of T24. Furthermore, ATCC karyotypes of the two lines show two shared-marker chromosomes. Thus, ECV-304 mopst probably is a derivative of T24, a line that was developed a couple of years earlier. It is important to emphasize that all stocks of ECV show similar properties, not just those from ATCC. It is clear that the contamination took place before the cell banks independently received initial stocks. Independent laboratories have reported that ECV showed some biomarkers expected of endothelial cell lines, namely Factor VIII, tubule formation on Matrigel, and/or Weibel-Palade bodies. Studies to determine if T24 exhibits any such markers are underway. |
| Culture Medium: | Medium 199 supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.25% trypsin/0.03% EDTA solution, rinse and remove trypsin. Allow the flask to sit at room temperature (or incubate at 37°C) until the cells detach (usually 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:6 to 1:10 is recommended |
| Fluid Renewal: | 2 to 3 times per week |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in BALB/c nu/nu mice |
References: Takahashi K et al. Spontaneous transformation and immortalization of human endothelial cells. In Vitro Cell. Dev. Biol. 26: 265-274, 1990. Takahashi K and Sawasaki Y. Human endothelial cell line, ECV304, produces pro-urokinase [letter]. In Vitro Cell. Dev. Biol. 27A: 766-768, 1991. Takahasi K and Sawasaki Y. Rare spontaneously transformed human endothelial cell line provides useful research tool [letter]. In Vitro Cell. Dev. Biol. 28A: 380-382, 1992. | |
