CADO-ES1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
CADO-ES1 |
Humane Ewing's Sarkom Zell-Linie |
300127 |
cryovial |
315,00 |
CADO-ES1 |
Humane Ewing's Sarkom Zell-Linie |
330127 |
vital |
395,00 |
| Designation: | CADO-ES1 |
| Depositor: | Kodama |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Japanese |
| Age/Stage: | 19 years |
| Gender: | female |
| Tissue: | Bone |
| Morphology: | Small round tumor cells |
| Celltype: | Ewing's sarcoma |
| Growth Properties: | Monolayer, semi-adherent |
| Description: | Established from the malignant pleural effusion of a 19 years old Japanese. |
| Culture Medium: | RPMI medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.1% trypsin/0.02% EDTA solution (final concentration), and let culture sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 6 days |
| Split Ratio: | A ratio of 1:3 to 1:5 is recommended |
| Fluid Renewal: | 1-2 times weekly |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma (DAPI, PCR), bacteria and fungi were negative |
| Biosafety Level: | 1 |
References: Franzius C, Kopka K, van Valen F, Riemann B, Sciuk J, Schober O. 3-(123I) Iodo-L-a-methyl tyrosine transport into human fibroblasts and comparison with Ewing's sarcoma cells. Nuclear Medicine and Biol 29(4): 483-490, 2002. Sonnemann J, Eckervogt V, Truckenbrod B, Boos J, Winkelmann W, van Valen F. The bisphosphonate pamidronate is a potent inhibitor of Ewing's sarcoma cell growth in vitro. Anticancer Drugs 14(9): 767-771, 2003. Gerth HU, Rompel A, Krebs B, Boos J, Lanvers-Kaminsky C. Cytotoxic effects of novel polyoxotungstates and a platinum compound on human cancer cell lines. Anticancer Drugs 16(1): 101-106, 2005. | |
