HOS
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HOS |
Humane Osteosarkom Zell-Linie |
300449 |
cryovial |
265,00 |
HOS |
Humane Osteosarkom Zell-Linie |
330449 |
vital |
335,00 |
| Designation: | HOS |
| Depositor: | Riken |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 13 years |
| Gender: | female |
| Tissue: | bone |
| Morphology: | A mixture of fibroblasts and epithelial like cells |
| Celltype: | sarcoma, osteogenic |
| Growth Properties: | monolayer |
| Description: | HOS cells exhibit a flat morphology, low saturation density, low plating efficiency in soft agar and are sensitive to chemical and viral transformation. The cells express alkaline phosphatase under basal conditions (Clover J, 1994). |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS supplemented with 2 mM L-glutamine, 1 mM non-essential amino acids (NEA) and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA solution. Add fresh 0.025% trypsin solution for 2 to 4 minutes at 37°C. Stop the enyme activity by adding fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Isoenzymes: | G6PD, B |
References: McAllister RM, Gardner MB, Greene AE, Bradt C, Nichols WW, Landing BH. Cultivation in vitro of cells derived from a human osteosarcoma. Cancer 27: 397-402, 1971. McAllister RM et al. Transformation and productive infection of human osteosarcoma cells by a feline sarcoma virus. Nat. New Biol. 230(17): 279-282, 1971. Rhim JS, Cho HY, Huebner RJ. Non-producer human cells by murine sarcoma virus. Int. J. Cancer 15(1): 23-29, 1975. Rhim JS et al. Characterization of non-producer human cells induced by Kirsten sarcoma virus. Int. J. Cancer 16: 840-849, 1975. Rhim JS. Characterization of sarcoma-positive, leukemia-negative (S+L-) human cells induced by the feline leukemia virus pseudotype of Moloney sarcoma virus. Proc. Soc. Exp. Biol. Med.167: 597-606, 1981. Rhim JS et al. Differential susceptibility of human cells to transformation by murine and avian sarcoma viruses. Proc. Soc. Exp. Biol. Med. 170: 350-358, 1982. Clover J, Gowen M. Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblastic phenotype? Bone 15(6): 585-591, 1994. Yee A et al. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J. Biol. Chem. 271: 471-477, 1996. Sunjic SB et al. The influence of 4-hydroxy-2-nonenal on proliferation, differentiation and apoptosis of human osteosarcoma cells. Biofactors 24(1): 141-148, 2005. | |
