MG-63
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
MG-63 |
Humane Osteosarkom Zell-Linie |
300441 |
cryovial |
265,00 |
MG-63 |
Humane Osteosarkom Zell-Linie |
330441 |
vital |
335,00 |
| Designation: | MG-63 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 14 years of age |
| Gender: | Male |
| Tissue: | Bone |
| Morphology: | Fibroblast-like |
| Celltype: | Osteosarcoma |
| Growth Properties: | Monolayer |
| Description: | High levels of interferon production can be induced using polyinosinic - polycytidylic acid, cycloheximide and actinomycin D. |
| Culture Medium: | DMEM supplemented with 2 mM L-glutamine and 10% fetal bovine serum |
| Subculturing: | Remove medium and rinse with 0.02% EDTA (versene) solution. Add fresh 0.025% trypsin/0.02% EDTA (versene) solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, remove trypsin by centrifugation and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X / Y CSF1PO: 10/12 D13S317: 11/11 D16S539: 11/11 D5S818: 11/12 D7S820: 10/10 THO1: 9.3/9.3 TPOX: 8/11 vWA: 16/19 D3S1358: 15/17 D21S11: 28/29 D18S51: 13/13 Penta E: 12/12 Penta D: 11/13 D8S1179: 11/15 FGA: 20/21 CLS ∙ Cell Lines Service, 2009. |
| Receptors Expressed: | transforming growth factor beta (TGF beta, type I and type II) |
| Products: | interferon |
References: Billiau A et al. Human interferon: mass production in a newly established cell line, MG-63. Antimicrob Agents Chemother 12: 11-5, 1977. Heremans H et al. In vitro cultivation of human tumor tissues. II. Morphological and virological characterization of three cell lines. Oncology 35: 246-52, 1978. Clover J et al. Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblastic phenotype? Bone 15: 585-591, 1994. Takeuchi Y et al. Relationship between actions of transforming growth factor (TGF)-beta and cell surface expression of its receptors in clonal osteoblastic cells. J Cell Physiol 162: 315-21, 1995. Yee A et al. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J Biol Chem 271: 471-477, 1996. Vial D et al. Coordinate regulation of fibronectin matrix assembly by the plasminogen activator system and vitronectin in human osteosarcoma cells. Cancer Cell Int 6: 8, 2006. Wiontzek M et al. Effects of dexamethasone and celecoxib on calcium homeostasis and expression of cyclooxygenase-2 mRNA in MG-63 human osteosarcoma cells. Clin Exp Rheumatol 24: 366-72, 2006. | |
