MHH-ES1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
MHH-ES1 |
Humane Ewing's Sarkom Zell-Linie |
300136 |
cryovial |
375,00 |
MHH-ES1 |
Humane Ewing's Sarkom Zell-Linie |
330136 |
vital |
465,00 |
| Designation: | MHH-ES1 |
| Depositor: | Hartmann |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Turkish |
| Age/Stage: | 12 years |
| Gender: | male |
| Tissue: | Bone |
| Morphology: | Small round tumor cells |
| Celltype: | Ewing's sarcoma |
| Growth Properties: | Monolayer, clusters |
| Description: | Established from the malignant ascites (with peritoneal metastasis). |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum |
| Subculturing: | Remove medium, add fresh 0.025% trypsin/0.02% EDTA solution for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 2 to 3 days. |
| Split Ratio: | A ratio of 1:3 to 1:5 is recommended |
| Fluid Renewal: | 1-2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
References: Wisniewski D et al. Characterization of potent inhibitors of the Bcr-Abl and the c-Kit receptor tyrosine kinase. Cancer Res 62: 4244-55, 2002. Kaufmann B et al. Structural variability of BM-40/SPAR/osteonectin glycosylation: implications for collagen affinity. Glycobiology 14: 609-19, 2004. | |
