MNNG-HOS
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
MNNG-HOS |
Humane Osteosarkom Zell-Linie |
300289 |
cryovial |
265,00 |
MNNG-HOS |
Humane Osteosarkom Zell-Linie |
330289 |
vital |
335,00 |
| Designation: | MNNG-HOS (Cl #5) |
| Depositor: | Rhim |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 13 years old |
| Gender: | female |
| Tissue: | Bone |
| Celltype: | Osteosarcoma |
| Growth Properties: | monolayer |
| Description: | This line was derived from HOS cells by transformation with 0.01 µg/ml MNNG (a carcinogenic nitrosamine). The cells exhibit a high saturation density, a high plating efficiency in soft agar and are tumorigenic in nude mice. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS supplemented with L-glutamine, 1% non-essential amino acids and 10% fetal bovine serum |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Isoenzymes: | G6PD, B |
References: Rhim JS et al. Transformation of human cells in culture by N-methyl-N'-nitro-N- nitrosoguanidine. Nature 256: 751-3, 1975. Rhim JS et al. Characterization of human cells transformed in vitro by N-methyl-N'- nitro-N-nitrosoguanidine. Int J Cancer 19: 505-10, 1977. Cooper CS et al. Charaterization of human transforming genes from chemically transformed, teratocarcinoma, and pancreatic carcinoma cell lines. Cancer Res 44: 1-10, 1984. Zhu X et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc Natl Acad Sci USA 93: 6091-5, 1996. Luu HH et al. An orthotopic model of human osteosarcoma growth and spontaneous pulmonary metastasis. Clin Exp Metastasis 22: 319-29, 2005. Halmos G et al. Charcterization of receptors for growth hormone-releasing hormone in human osteosarcomas and Ewing's sarcomas. Int J Oncol 29: 463-9, 2006. | |
