RD-ES
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
RD-ES |
Humane Ewing's Sarkom Zell-Linie |
300410 |
cryovial |
265,00 |
RD-ES |
Humane Ewing's Sarkom Zell-Linie |
330410 |
vital |
335,00 |
| Designation: | RD-ES |
| Depositor: | Kirchen |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Age/Stage: | 19 years old |
| Gender: | male |
| Tissue: | Bone |
| Morphology: | epithelial |
| Celltype: | Ewing's sarcoma |
| Growth Properties: | adherent |
| Description: | The cell line was initiated by G. Marshall and M. Kirchen from a primary osseous Ewing's sarcoma of the humerus. Ultrastructurally the cells exhibit primitive cell junctions, possess glycogen pools and are 20 to 25 microns in diameter. The cells grow as a loosely attached monolayer in small clusters of 5 to 10 cells. |
| Culture Medium: | RPMI 1640 medium with sodium pyruvate, 85%; fetal bovine serum, 15% |
| Subculturing: | Shake the flask after removing most of the medium. Add fresh medium and transfer to new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:8 is recommended |
| Fluid Renewal: | 2 to 3 times per week |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X / Y CSF1PO: 11/11 D13S317: 11/12 D16S539: 9/11 D5S818: 11/11 D7S820: 10/10 THO1: 7/7 TPOX: 9/11 vWA: 17/17 D3S1358: 15/15 D21S11: 28/28 D18S51: 14/18 Penta E: 11/13 Penta D: 9/12 D8S1179: 13/13 FGA: 21/25 CLS ∙ Cell Lines Service, 2009. |
| Antigen Expression: | Blood type B; Rh+ |
| Isoenzymes: | G6PD, B; PGM1, 1-2; PGM3, 1; ES-D, 1; Me-2, 1-2; AK-1, 1; GLO-1, 1-2; Phenotype Frequency Product: 0.0359 |
References: Sano K et al. Expression of the smg p25A (a ras p21-like GTP-binding protein) gene in human neuroblastoma cell lines and tumor tissue. Cancer Res 50: 7242-5, 1990. Goji J et al. Chondrocytic d9fferentiation of peripheral neuroectodermal tumor cell line in nude mouse xenograft. Cancer Res 52: 4214-20, 1992. Hofbauer S et al. Insulin-like growth factor-I-dependent growth and in vitro chemosensitivity of Ewing's sarcoma and peripheral primitive neuroectodermal tumour cell lines. Eur J Cancer 29A: 241-5, 1993. Hotfilder M et al. PI3K/AKT is involved in mediating survival signals that rescue Ewing tumour cells from fibroblast growth factor 2-induced cell death. Br J Cancer 92: 705-10, 2005. Koepler O et al. Synthesis and DNA binding properties of novel benzo[b]isoquino[2,3-h]-naphthyridines. Org Biomol Chem 3: 2848-58, 2005. Kubo T et al. Inhibitory effects of a new bisphosphonate, minodronate, on proliferation and invasion of a variety of malignant bone tumor cells. J Orthop Res 24: 1138-44, 2006. | |
