SW-1353
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SW-1353 |
Humane Knochen Chondrosarkom Zell-Linie |
300440 |
cryovial |
265,00 |
SW-1353 |
Humane Knochen Chondrosarkom Zell-Linie |
330440 |
vital |
335,00 |
| Designation: | SW-1353 |
| Depositor: | Leibovitz |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 72 years |
| Gender: | female |
| Tissue: | bone (humerus) |
| Morphology: | fibroblast |
| Celltype: | chondrosarcoma |
| Growth Properties: | monolayer |
| Description: | The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian. The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics. |
| Culture Medium: | Leibovitz's L-15 medium supplemented with 1% L-glutamine and 10% fetal bovine serum |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Karyotype: | hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident |
| Antigen Expression: | Blood type O- |
| Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 2; ES-D, 2; AK-1, 1; GLO-1, 2; Phenotype Frequency Product: 0.00009 |
References: Merluzzi VJ et al. Membrane-associated interleukin 1 activity on human U937 tumor cells: stimulation of PGE2 production by human chondrosarcoma cells. J Immunol 139: 166-8, 1987. Ouyang P. An in vitro model to study mesenchymal-epithelial transformation. Biochem Biophys Res Commun 246: 771-6, 1998. Rodriques M et al. Comparative 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin uptake in human soft tissue sarcoma cell lines. Eur J Nucl Med. 27: 1839-43, 2000. Zenmyo M et al. p21 and parathyroid hormone-related peptide in the growth plate. Calcif Tissue Int 67: 378-81, 2000. Rodrigues M et al. Verapamil decreases accumulation of 99Tcm-MIBI and 99Tcm-tetrofosmin in human breast cancer and soft tissue sarcoma cell lines. Nucl Med Commun. 22: 645-50, 2001. Wu C et al. Furin-mediated processing of pro-C-type natriuretic peptide. J Biol Chem 278: 25847-52, 2003. Kubo T et al. Inhibitory effects of a new bisphosphonate, minodronate, on proliferation and invasion of a variety of malignant bone tumor cells. J Orthop Res 24: 1138-44, 2006. Ross S et al. High-content screening analysis of the p38 pathway: profiling of structurally related p38alpha kinase inhibitors using cell-based assays. Assay Drug Dev Technol 4: 397-409, 2006. | |
