KHOS-312H
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
KHOS-312H |
Humane Osteosarkom Zell-Linie |
300447 |
cryovial |
265,00 |
KHOS-312H |
Humane Osteosarkom Zell-Linie |
330447 |
vital |
335,00 |
| Designation: | KHOS-312H |
| Depositor: | J.S. Rhim |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 13 years |
| Gender: | female |
| Tissue: | Bone |
| Morphology: | fibroblast |
| Celltype: | osteogenic sarcoma |
| Growth Properties: | monolayer |
| Description: | The growth properties of KHOS-312H are similar to HOS (TE-85). The KHOS-312H does not represent a rescuable Kirsten murine sarcoma virus genome. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, 90%; fetal bovine serum, 10% |
| Subculturing: | Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, 90%; fetal bovine serum, 10% Fluid enewal: 2 to 3 times weekly Subculturing: Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at 37°C for 10 to 12 minutes or until cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 days. |
| Split Ratio: | A ratio of 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | no |
References: Cho HY et al. Revertants of human cells transformed by murine sarcoma virus. Science 194: 951-3, 1976. Yang YH et al. Reversion of Kirsten sarcoma virus transformed human cells: elimination of the sarcoma virus nucleotide sequences. J Gen Virol 43: 447-51, 1979. Cho HY et al. Cycloheximide-dependent reversion of human cells transformed by MSV and chemical carcinogen. Science 205: 691-3, 1979. | |
