BT-474
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
BT-474 |
Humane Brust Karzinom Zell-Linie |
300131 |
cryovial |
265,00 |
BT-474 |
Humane Brust Karzinom Zell-Linie |
330131 |
vital |
335,00 |
| Designation: | BT-474 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 60 years old |
| Gender: | female |
| Tissue: | Breast |
| Morphology: | epithelial |
| Celltype: | mammary gland |
| Growth Properties: | Monolayer; the cells form compact, multilayered colonies; growing slowly and rarely becoming confluent. |
| Description: | The BT-474 line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast. |
| Culture Medium: | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, 1.2 mM oxaloacetic acid, 0.02 mg/ml bovine insulin, 10% NCTC 135 medium and 10% fetal bovine serum. Alternatively, DMEM:Ham's F12 (1:1 mixture) supplemented with 2 mM L-glutamine and 10% fetal bovine serum may be used. |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin/0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, remove trypsin by centrifugation, and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 to 1:3 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Karyotype: | mode = 55; range = 50 to 112; bimodal shift 58 - 59 and 100 in later passages with 3 marker chromosomes |
| Isoenzymes: | G6PD, B; PGM3, 1; PGM1, 1; ES-D, 1; Me-2, 0; AK-1, 1; GLO-1, 1; Phenotype Frequency Product: 0.0426 |
| Viruses: | Test for SMR-Provirus: env-gene negative / gag-gene negative |
| Virus Susceptibility: | mouse mammary tumor virus (RIII-MuMTV) |
References: Lasfargues EY et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J Natl Cancer Inst 61: 967-78, 1978. Lasfargues EY et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-9, 1979. Imai Y et al. Epidermal growth factor receptors and effect of epidermal growth factor on growth of human breast cancer cells in long-term tissue culture. Cancer Res 42: 4394-8, 1982. Kumar R et al. Regulation of phosphorylation of the c-erbB-2/HER2 gene product by a monoclonal antibody and serum growth factor(s) in human mammary carcinoma cells. Mol Cell Biol 11: 979-86, 1991. Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res 57: 5386-90, 1997. Wong LJ et al. AIB1 gene amplification and the instability of polyQ encoding sequence in breast cancer cell lines. BMC Cancer 6: 111, 2006. Isaza-Molina MA et al. In vitro effects of bovine dialyzable leukocyte extract (bDLE) in cancer cells. Cytotherapy 8: 408-14, 2006. Liang Y et al. Vascular endothelial growth factor induces proliferation of breast cancer cells and inhibits the anti-proliferative activity of anti-hormones. Endocr Relat Cancer 13: 905-19, 2006. | |
