BT-549
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
BT-549 |
Humane Brust Karzinom Zell-Linie |
300132 |
cryovial |
315,00 |
BT-549 |
Humane Brust Karzinom Zell-Linie |
330132 |
vital |
395,00 |
| Designation: | BT-549 |
| Depositor: | Coutinho |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 72 years |
| Gender: | female |
| Tissue: | Breast |
| Morphology: | epithelial |
| Celltype: | mammary gland |
| Growth Properties: | monolayer |
| Description: | The BT-549 line was isolated in 1978 by W.G. Coutinho and E.Y. Lasfargues. Source tissue consisted of a papillary, invasive ductal tumor which had metastasized to 3 of 7 regional lymph nodes. The established population was polymorphic with epithelial like components and multinucleated giant cells. A mucin-like material was secreted into the medium. |
| Culture Medium: | RPMI 1640 medium with 0.023 IU/ml insulin, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, add fresh 0.025% trypsin, 0.02% EDTA solution, swirl gently, remove trypsin and let the culture sit at 37°C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. When cultures become confluent some cells will slough off into the medium, these cells can be centrifuged and placed into new culture flasks. |
| Split Ratio: | A ratio of 1:2 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Biosafety Level: | 1 |
| Karyotype: | mode = 74; range = 53 to 140; three marker chromosomes |
| DNA Profile (STR): | Amelogenin: X / X CSF1PO: 10/12 D13S317: 11/11 D16S539: 8/8 D5S818: 11/11 D7S820: 9/10 THO1: 9,3/9,3 TPOX: 8/8 vWA: 15/15 D3S1358: 18/18 D21S11: 32.2/32.2 D18S51: 15/15 Penta E: 14/14 Penta D: 13/13 D8S1179: 14/16 FGA: 19/19 CLS ∙ Cell Lines Service, 2009. |
| Isoenzymes: | G6PD, B; PGM1, 2; PGM3, 1; ES-D, 1; Me-2, 1; AK-1, 1; GLO-1, 1-2; Phenotype Frequency Product: 0.0048 |
References: Katayose Y et al. Promoting apoptosis: a novel activity associated with the Cyclin-dependent kinase inhibitor p27. Cancer Res 57: 5441-5, 1997. Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res 57: 5386-90,1997. | |
