MA-CLS-2
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
MA-CLS-2 |
Humane Brust Karzinom Zell-Linie |
300271 |
cryovial |
375,00 |
MA-CLS-2 |
Humane Brust Karzinom Zell-Linie |
330271 |
vital |
465,00 |
| Designation: | MA-CLS-2 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 47 years |
| Gender: | female |
| Tissue: | mammary gland |
| Morphology: | epithelial |
| Celltype: | carcinoma-metastatic |
| Growth Properties: | monolayer |
| Description: | The MA-CLS-2 was established from the pleural effusion of a 47 year-old female in 1998 by CLS ∙ Cell Lines Service; pT1 NO GII. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution,remove trypsin and let the culture sit at room temperature (or at 37C)until the cells detach (about 10 minutes).Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
References: not published | |
