MDA-MB-231
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
MDA-MB-231 |
Humane Brust Karzinom Zell-Linie |
300275 |
cryovial |
265,00 |
MDA-MB-231 |
Humane Brust Karzinom Zell-Linie |
330275 |
vital |
335,00 |
| Designation: | MDA-MB-231 |
| Depositor: | Cailleau |
| Organism: | Human |
| Ethnicity: | Caucasian |
| Age/Stage: | 51 years of age |
| Gender: | female |
| Tissue: | Breast; mammary gland; (pleural effusion) |
| Celltype: | epithelial (adenocarcinoma) |
| Growth Properties: | monolayer |
| Culture Medium: | DMEM:Ham's F12 (1:1 mixture) supplemented with 2 mM L-glutamine and 5% fetal bovine serum. Incubate at 37°C/5% CO2. |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice as well as in ALS treated BALB/c mice; forms poorly differentiated adenocarcinoma (grade III) |
| Oncogene: | wnt3+; wnt7h+ |
| Karyotype: | mean chromosome number = 68; (P17), hypotriploid with abnormalities including breaks, secondary constrictions and fragmentations |
| Modal Number: | 65 |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO:12,13 D13S317:13 D16S539:12 D5S818:12 D7S820:8,9 TH01:7,9.3 TPOX:8,9 vWA:15,19 D3S1358: 16 D21S11: 30,33.2 D18S51: 11,16 Penta E: 11 Penta D: 11,14 D8S1179: 13 FGA: 22,23 |
| Antigen Expression: | Blood Type O; Rh- |
| Receptors Expressed: | epidermal growth factor (EGF); transforming growth factor alpha (TGF alpha) |
| Isoenzymes: | Me-2, 1-2; PGM3, 1; PGM1, 1-2; ES-D, 1; AK-1, 1; GLO-1, 2; G6PD B; Phenotype Frequency Product: 0.0229 |
References: Cailleau R et al. Breast tumor cell lines from pleural effusions. J Natl Cancer Inst 53: 661-74, 1974. Cailleau R et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-5, 1978. Siciliano MJ et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res 39: 919-22, 1979. Brinkley BR et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res 40: 3118-29, 1980. Satya-Prakash KL et al. Cytogenetic analysis on eight human breast tumor cell lines: high frequencies of 1q, 11q and HeLa-like marker chromosomes. Cancer Genet Cytogenet 3: 61-73, 1981. Bates SE et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. Dickstein B et al. Increased epidermal growth factor receptor in an estrogen-responsive, adriamycin-resistant MCF-7 cell line. J Cell Physiol 157: 110-8, 1993. Huguet EL et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res 54: 2615-21, 1994. Soker S et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J Biol Chem 271: 5761-7, 1996. Sheng S et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc Natl Acad Sci USA 93: 11669-74, 1996. Katayose Y et al. Promoting apoptosis: a novel activity associated with the Cyclin-dependent kinase inhibitor p27. Cancer Res 57: 5441-5, 1997. Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res 57: 5386-90, 1997. De Vincenzo R et al. Antiproliferative activity of colchicine analogues on MDR-positive and MDR-negative human cancer cell lines. Anticancer Drug Des 13: 19-33, 1998. Jiang WG et al. Expression of membrane type-1 matrix metalloproteinase, MT1-MMP in human breast cancer and its impact on invasiveness of breast cancer cells. Int Mol Med 17: 583-90, 2006. Maclaughlin BW et al. Effects of homeopathic preparations on human prostate cancer growth in cellular and animal modls. Integr Cancer Ther 5: 362-72, 2006. | |
