MDA-MB-435S
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
MDA-MB-435S |
Humane Brust Karzinom Zell-Linie |
300277 |
cryovial |
265,00 |
MDA-MB-435S |
Humane Brust Karzinom Zell-Linie |
330277 |
vital |
335,00 |
MDA-MB-435_Melanocytic origin
MDA-MB-435s_Ellison.pdf (191KB)
MDA-MB-435s_Ellison.pdf
| Designation: | MDA-MB-435S |
| Depositor: | Cailleau |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 31 years of age |
| Gender: | female |
| Tissue: | Breast; mammary gland (pleural effusion) / Skin? |
| Morphology: | spindle shaped |
| Celltype: | ductal carcinoma or melanocytic? |
| Growth Properties: | monolayer |
| Description: | MDA-MB-435S is a spindle shaped strain which evolved from the parent line (435) isolated in 1976 (R. Cailleau et al.) from the pleural effusion of a 31 year old female with metastatic ductal adenocarcinoma of the breast. Parental cells displayed the dispersed pattern (type II) when treated with immunofluorescent stain for tubulin. Recent findings point to a rather melanocytic origin of this cell line (Ellison 2002). |
| Culture Medium: | DMEM:Ham's F12 supplemented with 2 mM L-glutamin and 10% fetal bovine serum. Incubate at 37°C/5%CO2. |
| Subculturing: | Remove medium and rinse with fresh 0.02% EDTA solution. Add fresh trypsin (0.025%)/EDTA (0.03%) solution, rinse and remove. Place flask at 37°C until the cells detach. Add fresh medium, carefully resuspend the cells and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | no |
| Karyotype: | modal number = 64 |
| DNA Profile (STR): | Amelogenin: X / X CSF1PO: 11/11 D13S317: 12/12 D16S539: 13/13 D5S818: 12/12 D7S820: 8/10 THO1: 6/7 TPOX: 8/11 vWA: 16/18 D3S1358: 14/14 D21S11: 30/30 D18S51: 13/17 Penta E: 10/12 Penta D: 9/11 D8S1179: 13/13 FGA: 12/21 CLS ∙ Cell Lines Service, 2009. |
| Isoenzymes: | G6PD, B; PGM1, 2; PGM3, 1; ES-D, 1; AK-1, 1; GLO-1, 2; Phenotype Frequency Product: 0.0071 |
| Products: | tubulin; actin |
References: Cailleau R et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-5, 1978. Siciliano MJ et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-22, 1979. Brinkley BR et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res 40: 3118-29, 1980. Sheng S et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc Natl Acad Sci USA 93: 11669-74, 1996. Zhu X et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc Natl Acad Sci USA 93: 6091-5, 1996. Ellison G et al. Further evidence to support the melanocytic origin of MDA-MB-435. J Clin Pathol: Mol Pathol 55:294–299, 2002. Sellappan S et al. Lineage infidelity of MDA-MB-435 cells: expression of melanocyte proteins in a breast cancer cell line. Cancer Res 64: 3479-85, 2004. Song L et al. Kinetics and mechanisms of activation of alpha-amino acid ester prodrugs of campothecins. J Med Chem 49: 4344-55, 2006. Leuschner C et al. LHRH-conjugated magnetic iron oxide nanoparticles for detection of breast cancer metastases. Breast Cancer Res Treat 99: 163-76, 2006. | |
