MDA-MB-436
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
MDA-MB-436 |
Humane Brust Adenokarzinom Zell-Linie |
300278 |
cryovial |
265,00 |
MDA-MB-436 |
Humane Brust Adenokarzinom Zell-Linie |
330278 |
vital |
335,00 |
| Designation: | MDA-MB-436 |
| Depositor: | Cailleau |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 43 years of age |
| Gender: | female |
| Tissue: | Breast; mammary gland; pleural effusion |
| Morphology: | pleomorphic with multinucleated component cells |
| Celltype: | Adenocarcinoma |
| Growth Properties: | monolayer |
| Description: | The line is pleomorphic and most cells react intensely with anti tubulin antibody as demonstrated by indirect immunofluorescence staining. |
| Culture Medium: | DMEM:Ham's F12 supplemented with 2 mM L-glutamin and 10% fetal bovine serum. Incubate at 37°C/5% CO2. |
| Subculturing: | Subcultures are prepared by scraping. Remove medium, add 3 to 5 ml of fresh medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | no |
| Karyotype: | modal number = 45 |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 12 D13S317: 10 D16S539: 9 D5S818: 13 D7S820: 10 TH01: 9.3 TPOX: 8 vWA: 14,20 D3S1358: 18 D21S11: 30,31.2 D18S51: 12 Penta E: 10,12 Penta D: 9 D8S1179: 10,14 FGA: 24 CLS – Cell Lines Service 2010. |
| Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 1; ES-D, 1; Me-2, 1; AK-1, 1; GLO-1, 2; Phenotype Frequency Product: 0.0326 |
| Products: | tubulin; actin |
References: Cailleau R et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-5, 1978. Siciliano MJ et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res 39: 919-22, 1979. Brinkley BR et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res 40: 3118-29, 1980. Lan MS et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res 50: 2997-3001, 1990. Kulp KS et al. Essiac(®) and Flor-Essence (®) herbal tonics stimulate the in vitro growth of human breast cancer cells. Breast Cancer Res Treat 98: 249-59, 2006. Srirangam CM et al. Effects of HIV protease inhibitor ritonavir on Akt-regulated cell proliferation in breast cancer. Clin Cancer Res 12: 1883-96, 2006. | |
