T-47D
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
T-47D |
Humane Brust Karzinom Zell-Linie |
300353 |
cryovial |
265,00 |
T-47D |
Humane Brust Karzinom Zell-Linie |
330353 |
vital |
335,00 |
| Designation: | T-47D |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 54 years of age |
| Gender: | female |
| Tissue: | Breast; mammary gland (pleural effusion) |
| Morphology: | epithelial |
| Celltype: | ductal carcinoma |
| Growth Properties: | monolayer |
| Description: | The T-47 line was isolated by I. Keydar from the pleural effusion of an infiltrating ductal carcinoma of the breast. The differentiated epithelial sub-strain T-47D reportedly contains cytoplasmic junctions, receptors to 17--estradiol, other steroids and calcitonin. It will form colonies in soft agar. |
| Culture Medium: | RPMI 1640 medium (or DMEM:Ham's F12; 1:1 mixture) supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA (versene) solution. Add fresh 0.025% trypsin/0.02% EDTA (versene) solution, swirl gently, remove trypsin and let the culture sit at 37°C until the cells detach. Add fresh medium, resuspend the cells and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Doubling Time: | 32 hrs |
| Freeze Medium: | CM-1 (CLS Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Oncogene: | wnt3 +; wnt7h +; wnt7b+ |
| Karyotype: | mode = 66; dicentric and extra long submetacentric chromosomes |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO:11,13 D13S317:12 D16S539:10 D5S818:12 D7S820:11 TH01:7,6 TPOX:11 vWA:14 D3S1358: 15,17 D21S11: 28,31 D18S51: 17 Penta E: 7,14 Penta D: 10,12 D8S1179: 13 FGA: 23 CLS – Cell Lines Service 2010. |
| Receptors Expressed: | estradiol; steroids; calcitonin; androgen; progesterone; glucocorticoid; prolactin; estrogen |
| Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 1; ES-D, 2; Ak-1, 1; GLO-1, 1-2 |
References: Keydar I et al. Establishment and characterization of a cell line of human breast carcinoma origin. Eur J Cancer 15: 659-70, 1979. Shiu RP. Processing of prolactin by human breast cancer cells in long term tissue culture. J Biol Chem 255: 4278-81, 1980. Lamp SJ et al. Calcitonin induction of a persistent activated state of adenylate cyclase in human breast cancer cells (T-47D). J Biol Chem 256: 12269-74, 1981. Sher E et al. Whole-cell uptake and nuclear localization of 1,25-dihydroxy-cholecalciferol by breast cancer cells (T-47D) in culture. Biochem J 200: 315-20, 1981. Freake HC et al. 1,25-Dihydroxyvitamin D3 specifically binds to a human breast cancer cell line (T-47D) and stimulates growth. Biochem Biophys Res Commun 101: 1131-8, 1981. Judge SM et al. Progesterone-specific stimulation of triglyceride biosynthesis in a breast cancer cell line (T-47D). Cancer Res 43: 4407-12, 1983. Faust JB et al. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res 52: 2460-3, 1992. Huguet EL et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res 54: 2615-21, 1994. Burfeind P et al. Antisense RNA to the type I insulin-like growth factor receptor suppresses tumor growth and prevents invasion by rat prostate cancer cells in vivo. Proc Natl Acad Sci USA 93: 7263-8, 1996. Bellet D et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res 57: 516-23, 1997. Hoppe HC et al. Identification of phosphatidylinositol mannoside as a mycobacterial adhesin mediating both direct and opsonic binding to nonphagocytic mammalian cells. Infect Immun 65: 3896-905, 1997. Langenheim JF et al. Development of a prolactin erceptor-targeting fusion toxin using a prolactin antagonist and a recombinant form of Pseudomonas exotoxin A. Breast Cancer Res Treat 90: 281-93, 2005. Gizard F et al. TReP-132 controls cell proliferation by regulating the expression of the cyclin-dependent kinase inhibitors p21WAF1/Cip1 and p27Kip1. Mol Cell Biol 25: 4335-4348, 2005. Werner HM et al. Apoptosis and proliferation in breast cancer cells, cultured in vitro: effects of SERMs. Climacteric 8: 294-299, 2005. Asaga S et al. Identification of a new breast cancer-related gene by restriction landmark genomic scanning. Anticancer Res 26: 35-42, 2006. Barnard RJ et al. Effects of a low-fat, high-fiber diet and exercise program on breast cancer risk factors in vivo and tumor cell growth and apoptosis in vitro. Nutr Cancer 55: 28-34, 2006. | |
