HEL-92.1.7
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HEL-92.1.7 |
Humane Erythroblasten Leukämie Zell-Linie |
300462 |
cryovial |
265,00 |
HEL-92.1.7 |
Humane Erythroblasten Leukämie Zell-Linie |
330462 |
vital |
335,00 |
| Designation: | HEL 92.1.7 |
| Depositor: | Martin |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 30 years |
| Gender: | male |
| Tissue: | Bone marrow |
| Morphology: | lymphoblast |
| Celltype: | erythroblast; erythroleukemia |
| Growth Properties: | suspension |
| Description: | These cells differentiate spontaneously into erythroblast-like cells. Macrophage-like differentiation can be induced with phorbol esters such as TPA (12-O- tetradecanoyl-phorbol-13-acetate) and PMA (phorbol myristic acid). |
| Culture Medium: | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10% |
| Subculturing: | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Antigen Expression: | HLA A3, Aw32, Bw35; Ia+ |
| Products: | hemoglobin; globin (G gamma, A gamma, epsilon, eta and alpha chains); beta-2-microglobulin; glycophorin |
References: Martin P and Papayannopoulou T. HEL cells: a new human erythroleukemia cell line with spontaneous and induced globin expression. Science 216: 1233-1235, 1982. Papayannopoulou T et al. Human erythroleukemia cell line (HEL) undergoes a drastic macrophage- like shift with TPA. Blood 62: 832-845, 1983. Gan W and Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. Riewald M et al. Identification and characterization of the cytoplasmic antiproteinase (CAP) in human platelets. J. Biol. Chem. 271: 7160-7167, 1996. Clark RA et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. | |
