HL-60
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HL-60 |
Humane Akute myelozytische Leukämie (AML) Zell-Linie |
300209 |
cryovial |
265,00 |
HL-60 |
Humane Akute myelozytische Leukämie (AML) Zell-Linie |
330209 |
vital |
335,00 |
| Designation: | HL-60 |
| Depositor: | Gallagher |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 36 years |
| Gender: | female |
| Tissue: | Blood, peripheral |
| Morphology: | lymphoblast |
| Celltype: | acute promyelocytic leukemia |
| Growth Properties: | suspension |
| Description: | HL-60 cells spontaneously differentiate. Differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Cultures can be maintained by addition or replacement of medium. Start new cultures at 2 x 105 viable cells/ml and subculture at 1 x 106 cells/ml. |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS - Cell Lines Service) |
| Sterility: | Negative for mycoplasma: DAPI Negative for bacteria and fungi: Thioglycollate, CASO-Bouillon EP |
| Biosafety Level: | 1 |
| Oncogene: | myc + |
| DNA Profile (STR): | Amelogenin: X/X CSF1PO: 13/14 D13S317: 8/11 D16S539: 11/11 D5S818: 12/12 D7S820: 11/12 THO1: 7/8 TPOX: 8/11 vWA: 16/16 D3S1358: 16/16 D21S11: 30/30 D18S51: 14/14 Penta E: 13/13 Penta D: 10/12 D8S1179: 13/13 FGA: 22/24 CLS ∙ Cell Lines Service, 2009. |
| Receptors Expressed: | complement; Fc |
| Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 1; ES-D,1; Me-2, 1; AK-1, 1; GLO-1, 1 |
| Reverse Transcriptase: | negative |
| Products: | tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid |
References: Collins SJ et al. Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture. Nature 270:347-349, 1977. Gallagher R et al. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood 54: 713-733, 1979. Mullineaux PM et al. Human tumor necrosis factor. Production,purification, and characterization. J. Biol. Chem. 260: 2345-2354, 1985. Tiffany HL et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J. Biol. Chem. 271: 12387-12393, 1996. Chan YJ et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. J. Virol. 70: 8590-8605, 1996. Mao M et al. RIG-E, a human homolog of the murine Ly-6 family, is induced by retinoic acid during the differentiation of acute promyelocytic leukemia cell. Proc. Natl. Acad. Sci. USA 93: 5910-5914, 1996. Lepley RA et al. Tyrosine kinase activity modulates catalysis and translocation of cellular 5-lipoxygenase. J. Biol. Chem. 271: 6179-6184, 1996. Chen HM et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. Nahm MH et al. Identification of cross-reactive antibodies with low opsonophogocytic activity for Streptoccus pneumoniae. J. Infect. Dis. 176: 698-703, 1997. Berninghausen O and Leippe M. Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica. Infect. Immun. 65: 3615-3621, 1997. Aparicio CL et al. Correction for label leakage in fluorimetric assays of cell adhesion. Biotechniques 23: 1056-1060, 1997. Mansat V et al. The protein kinase C activators phorbol esters and phosphatidylserine inhibit neutral phingomyelinase activation, ceramide generation, and apoptosis triggered by daunorubicin. Cancer Res. 57: 5300-5304, 1997. Cuthbert JA and Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. Michael JM et al. Resistance to radiation-induced apoptosis in Burkitt's lumphoma cells is associated with defective ceramide signaling. Cancer Res. 57: 3600-3605, 1997. Clark RA et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. | |
