HSB
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HSB |
Humane T-Zell-Lymphom Zell-Linie |
300214 |
cryovial |
265,00 |
HSB |
Humane T-Zell-Lymphom Zell-Linie |
330214 |
vital |
335,00 |
| Designation: | HSB |
| Depositor: | Foley |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 11.5 years |
| Gender: | male |
| Tissue: | blood, peripheral |
| Morphology: | lymphoblast |
| Celltype: | leukemia, acute lymphoblastic; T-lymphoblast |
| Growth Properties: | suspension |
| Description: | Derived from the same buffy coat preparation as CCL-120 (CCRF-SB) by serially transplanting into newborn syrian hamsters. |
| Culture Medium: | Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10% - OR - RPMI 1640 medium, 90%; fetal bovine serum, 10% |
| Subculturing: | Cultures can be maintained by addition or replacement of medium. Establish new cultures at 5 X 10 exp5 viable cells/ml. |
| Fluid Renewal: | Add fresh medium (10 to 20% by volume) every 3 to 4 days |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Antigen Expression: | HLA A1, A2, B12, B17, Cw2; CD5 (78%), CD7 (96%) |
| Isoenzymes: | G6PD, B |
| Reverse Transcriptase: | negative |
References: M.D. Anderson Hospital and Tumor Inst. Monograph. 21, 1967. Proc. Am. Assoc. Cancer Res. 8: 1, 1967. Adams RA et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121, 1968. | |
