Jurkat
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
Jurkat |
Humane Akute Lymphozytische Leukämie (ALL) Zell-Linie |
300223 |
cryovial |
315,00 |
Jurkat |
Humane Akute Lymphozytische Leukämie (ALL) Zell-Linie |
330223 |
vital |
395,00 |
| Designation: | Jurkat clone E6-1 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Gender: | Male |
| Tissue: | Leukemia, acute T cell; T lymphocyte |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 105 cells/ml and maintain between 1 x 105 and 1 x 106 cells/ml. Do not allow the cell concentration to exceed 1 X 106 cells/ml. |
| Fluid Renewal: | 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Karyotype: | modal number = 46; range = 41 to 47; the karyotype is 46,XY,-2,-18, del(2)(p21p23), del(18)(p11.2) |
| Antigen Expression: | CD3 |
References: Gillis S and Watson J. Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line. J Exp Med 152: 1709-19, 1980. Weiss A et al. The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL-2 production reflects events occurring at a pre-translational level. J Immunol 133: 123-8, 1984. Churchill MJ et al. The rev-responsive element negatively regulates human immunodeficiency virus type 1 env mRNA expression in primate cells. J Virol 70: 5786-90, 1996. Kolanus W et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-42, 1996. Gan W and Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J Biol Chem 271: 623-6, 1996. Tiffany HL et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J Biol Chem 271: 12387-93, 1996. Chan YJ et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. J Virol 70: 8590-605, 1996. Kung SH and Medveczky PG. Identification of a herpesvirus saimiri cis-acting DNA fragment that permits stable replication of episomes in transformed T cells. J Virol 70: 1738-44, 1996. Bloom TJ and Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc Natl Acad Sci USA 93: 14188-92, 1996. Li YM et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc Natl Acad Sci USA 93: 11047-52, 1996. Linette GP et al. Cross talk between cell death and cell cycle progression: BCL-2 regulates NFAT-mediated activation. Proc Natl Acad Sci USA 93: 9545-52, 1996. Miranda L et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc Natl Acad Sci USA 93: 7695-700, 1996. Yang RY et al. Expression of galectin-3 modulates T-cell growth and apoptosis. Proc Natl Acad Sci USA 93: 6737-42, 1996. Gibson S et al. Functional LCK is required for optimal CD28-mediated activation of the TEC family tyrosine kinase EMT/ITK. J Biol Chem 271: 7079-83, 1996. Ponton A et al. The CD95 (APO-1/Fas) receptor activates NF-kappaB independently of its cytotoxic function. J Biol Chem 271: 8991-5, 1996. August A and Dupont B. Association between mitogen-activated protein kinase and the zeta chain of the T cell receptor (TcR) with the SH2,3 domain of p56lck. J Biol Chem 271: 10054-9, 1996. Kotanides H and Reich NC. Interleukin-4-induced STAT6 recognizes and activates a target site in the promoter of the interleukin-4 receptor gene. J Biol Chem 271: 25555-61, 1996. Hartley D and Corvera S. Formation of c-Cb1-phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism. J Biol Chem 271: 21939-43, 1996. Chen HM et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J Biol Chem 271: 15743-52, 1996. Berninghausen O and Leippe M. Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica. Infect Immun 65: 3615-21, 1997. El-Ali J et al. Cell stimulus and lysis in a microfluidic device with segmented gas-liquid flow. Anal Chem 77: 3629-36, 2005. Borner C et al. Cannabinoid receptor type 2 agonists induce transcription of the µ-opioid receptor gene in Jurkat T cells. Mol Pharmacol 69: 1486-91, 2006. McCreary CR et al. Real-time measurement of cytosolic free calcium concentration in Jurkat cells during ELF magnetic field exposure and evaluation of the role of cell cycle. Bioelectromagnetics 27: 354-64, 2006. | |
