K562
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
K562 |
Humane Akute Myelozytische Leukämie Zell-Linie |
300224 |
cryovial |
265,00 |
K562 |
Humane Akute Myelozytische Leukämie Zell-Linie |
330224 |
vital |
335,00 |
| Designation: | K-562 |
| Depositor: | Lozzio |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 53 years |
| Gender: | female |
| Tissue: | bone marrow |
| Morphology: | lymphoblast |
| Celltype: | chronic myelogenous leukemia |
| Growth Properties: | suspension |
| Description: | The cells spontaneously differentiate into precursors of the erythroid, granulocytic and monocytic series. The line is EBNA negative. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 x 105 viable cells/ml. Subculture at 1 x 106 cells/ml. |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 2 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 9,10 D13S317: 8 D16S539: 11,12 D5S818: 11,12 D7S820: 9,11 THO1: 9.3 TPOX: 8,9 vWA: 16 D3S1358: 16 D21S11: 29,30 D18S51: 15 Penta E: 5,14 Penta D: 9,13 D8S1179: 12 FGA: 21,24 CLS ∙ Cell Lines Service, 2010. |
| Antigen Expression: | CD7 (25%) |
| Isoenzymes: | G6PD, B; AK-1, 1; ES-D, 1; GLO-1, 2; PGM1, 0; PGM3, 1; Me-2, 0 |
| Reverse Transcriptase: | negative |
| Viruses: | Tested positive for SMRV (Squirrel Monkey RetroVirus) by PCR |
References: Lozzio CB and Lozzio BB. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45: 321-334, 1975. Ortaldo JR et al. Specificity of natural cytotoxic reactivity of normal human lymphocytes against a myeloid leukemia cell line. J Natl Cancer Inst 59: 77-82, 1977. Lozzio BB and Lozzio CB. Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Leuk Res 3: 363-370, 1979. Andersson LC et al. K562--a human erythroleukemic cell line. Int J Cancer 23: 143-7, 1979. Koeffler HP and Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-50, 1980. Lozzio BB et al. A multipotential leukemia cell line (K-562)of human origin. Proc Soc Exp Biol Med 166: 546-50, 1981. Dimery IW et al. Variation amongst K562 cell cultures. Exp Hematol 11: 601-10, 1983. Chan YH et al. Two distinct upstream regulatory domains containing multicopy cellular transcription factor binding sites provide basal repression and inducible enhancer characteristics to the immediate-early IES (US3) promoter from human cytomegalovirus. J Virol 70: 5312-28, 1996. Kolanus W et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-42, 1996. Gan W and Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J Biol Chem 271: 623-6, 1996. Tiffany HL et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J Biol Chem 271: 12387-93, 1996. Chan YJ et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. J Virol 70: 8590-605, 1996. Nauseef WM et al. Effect of the R569W missense mutation on the biosynthesis of myeloperoxidase. J Biol Chem 271: 9546-9, 1996. Grune T et al. Degradation of oxidized proteins in K562 human hematopoietic cells by proteasome. J Biol Chem 271: 15504-9, 1996. Fortin H et al. In vivo antitumor activity of clitocine , an exocyclic amino nucleoside isolated from Lepista inverse. ChemMedChem 1: 189-196, 2006. Vasilev N et al. Production of justicidin B, a cytotoxic arylnaphthalene lignan from genetically transformed root cultures of Linum leonii. J Nat Prod 69: 1014-7, 2006. Momekov G et al. Cytoprotective effects of 5 benzophenones and xanthone from Hypericum annulatum in models of epirubicin-induced cytotoxicity: SAR-analysis and mechanistic investigations. Med Chem 2: 377-84, 2006. | |
