KG-1A
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
KG-1A |
Humane Akute myelozytische Leukämie (AML) Zell-Linie |
300234 |
cryovial |
315,00 |
KG-1A |
Humane Akute myelozytische Leukämie (AML) Zell-Linie |
330234 |
vital |
395,00 |
| Designation: | KG-1A |
| Depositor: | D.W. Golde |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 59 years |
| Gender: | male |
| Tissue: | Bone marrow |
| Morphology: | myeloblast |
| Celltype: | Acute myelogenous leukemia (AML) |
| Growth Properties: | Suspension |
| Description: | The KG-1A cell line is derived from the KG-1 cell line and is almost identical. These cells do not spontaneously differentiate to granulocyte and macrophage like cells, do not express DR and do not respond to colony stimulating factor (CSF). The line is EBNA negative (EBNA-). |
| Culture Medium: | Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 80%; fetalbovine serum, 20% |
| Subculturing: | Subculture by centrifugation with a 1:2 division of the cell pellet. Optimal cell density is no less than 1 X 10 exp5 cells/ml and no more than 1 X 10 exp6 cells/ml. |
| Split Ratio: | A ratio of 1:2 is recommended |
| Fluid Renewal: | twice weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Antigen Expression: | HLA A30, A31, B35, Cw4 |
| Isoenzymes: | G6PD, B; PGM1, 1-2; PGM3, 0; ES-D, 1; Me-2, 1; AK-1, 0; GLO-1, 2 |
| Reverse Transcriptase: | negative |
References: Koeffler HP et al. An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1). Blood 56: 265-73, 1980. Koeffler HP. Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications. Blood 62: 709-21, 1983. Chen H et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J Biol Chem 271: 15743-52, 1996. Clark RA et al. Tenascin supports lymphocyte rolling. J Cell Biol 137: 755-65, 1997. Walter RB et al. Influence of CD33 expression levels and ITIM-dependent internalization on gemtuzumab ozogamicin-induced cytotoxicity. Blood 105: 1295-302, 2005. Rutella S et al. Granulocyte colony-stimulating factor enhances the in vitro cytotoxicity of gemtuzumab ozogamicin againt acute myeloid leukaemia cell lines and primary blast cells. Exp Hematol 34: 54-65, 2006. | |
