REH
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
REH |
Humane lymphoblastoide Leukämie Zell-Linie |
300320 |
cryovial |
265,00 |
REH |
Humane lymphoblastoide Leukämie Zell-Linie |
330320 |
vital |
335,00 |
| Designation: | REH |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Tissue: | Hematopoiesis |
| Morphology: | lymphoblast |
| Celltype: | acute lymphocytic leukemia (non-T; non-B); pre B-ALL. |
| Growth Properties: | suspension |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM-L-glutamine and 10% fetal bovine serum |
| Subculturing: | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/ml and maintain between 2 X 105 and 1 X 106 cells/ml. |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Antigen Expression: | CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%), CD20+, |
| Immunology: | HLA-DR+, CALLA+ |
References: Bensimon J et al. X-ray sensitivity of human cultivated lymphoblastic cells in media enriched in nickel sulphate. CR Acad Sci Hebd Seances Acad Sci D 284: 867-9, 1977. Rosenfeld C et al. An effect human leukaemic cell line: REH. Eur J Cancer 13: 377-9, 1977. Rosenfeld C et al. Phenotypic characterisation of a unique non-T, non-B acute lymphoblastic leukaemia cell line. Nature 267: 841-3, 1977. Billard C et al. Serum-controlled membrane dynamic changes in growing human leukemia and lymphoma cells. Cancer Biochem Biophys 6: 149-56, 1983. Martyre MC et al. Modulation of CALLA and HLA-DR on a non T non B leukemic cell line by lipid treatment. Bio Cell 52: 243-8, 1984. Behr S et al. Engaging CD19 or target of an antiproliferative antibody 1 on human B lymphocytes induces binding of B cells to the interfollicular stroma of human tonsils via Integrin a4/b1 and Fibronectin. J Exp Med 182: 1191-9, 1995. Golay J et al. The sensitivity of a acute lymphoblastic leukaemia cells carrying the t(12;21) translocation to campath-1H-mediated cell lysis. Haematologica 91: 322-30, 2006. Leclerc GJ et al. Analysis of folypoly-gamma-glutamate synthetase gene expression in human B-precursor ALL and T-lineage ALL cells. BMC Cancer 6: 132, 2006. | |
