RPMI-1788
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
RPMI-1788 |
Humane B-Lymphozyten Zell-Linie |
300318 |
cryovial |
265,00 |
RPMI-1788 |
Humane B-Lymphozyten Zell-Linie |
330318 |
vital |
335,00 |
| Designation: | RPMI 1788 |
| Depositor: | Moore/Lindl |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 33 years |
| Gender: | male |
| Tissue: | blood, peripheral; leukocyte; B lymphocyte |
| Morphology: | lymphoblast |
| Growth Properties: | suspension |
| Description: | The cells are EBNA positive. |
| Culture Medium: | Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10% - OR - RPMI 1640 medium, 80%; fetal bovine serum, 20% |
| Subculturing: | Cultures can be maintained by addition or replacement of fresh medium. Cultures are established at 3 X 10 exp5 viable cells/ml. |
| Fluid Renewal: | Add fresh medium (10% to 20% by volume) every 3 to 4 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 2 |
| Karyotype: | human male; hypodiploid; stable |
| Antigen Expression: | HLA A2, Aw33, B7, B14 |
| Isotype: | IgM; lambda light chain |
| Isoenzymes: | G6PD, B |
| Reverse Transcriptase: | negative |
| Products: | immunoglobulin; lymphotoxin (LT) also known as tumor necrosis factor beta (TNF-beta, TNF beta) |
References: Huang CC et al. Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies. J Natl Cancer Inst 43: 1119-28, 1969. Calebaugh DL et al. A skin reactive factor elaborated from a human lymphoblastoid cell line (PRMI 1788). Cell Immunol 11: 286-303, 1974. Tan JC et al. Effects of radiation and porphyrin on mitosis and chromosomes in human hematopoietic cell lines. J Med 7: 169-76, 1976. Aggarwal BB et al. Human lymphotoxin. Production by a lymphoblastoid cell line, purification, and initial characterization. J Biol Chem 259: 686-91, 1984. Gray PW et al. Cloning and expression of cDNA for human lymphotoxin, a ymphokine with tumour necrosis activity. Nature 312: 721-4, 1984. Cointe D et al. Unusual N-glycosylation of a recombinant human erythropoietin expressed in a human lymphoblatoid cell line does not alter its biological properties. Glycobiology 10: 511-9, 2000. Khabar KS et al. Expressed gene clusters associated with cellular sensitivity and resistance towards anti-viral and anti-proliferative actions of interferon. J Mol Biol 342: 833-46, 2004. | |
