RPMI-8226
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
RPMI-8226 |
Humane Myelom Zell-Linie |
300431 |
cryovial |
265,00 |
RPMI-8226 |
Humane Myelom Zell-Linie |
330431 |
vital |
335,00 |
| Designation: | RPMI-8226 |
| Depositor: | Moore |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 61 years |
| Gender: | male |
| Tissue: | Blood |
| Morphology: | lymphoblast |
| Celltype: | Myeloma |
| Growth Properties: | monolayer/suspension |
| Description: | There is no evidence of heavy chain production (cytoplasmic or secreted). |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Cultures can be maintained by addition or replacement of fresh medium.Cultures can be established at 5 x 105 viable cells/ml. Maximum cell density at 1-2 x 106 cell/ml. |
| Freeze Medium: | CM-1 (CLS Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Antigen Expression: | HLA Aw19, B15, B37, Cw2 |
| Isotype: | lambda light chain |
| Isoenzymes: | G6PD, A |
| Reverse Transcriptase: | negative |
| Products: | immunoglobulin light chain |
References: Matsuoka Y et al. Production of free light chains of immunoglobulin by a hematopoietic cell line derived from a patient with multiple myeloma. Proc. Soc. Exp. Biol. Med. 125: 1246-1250, 1967. Moore GE, Kitamura H. Cell line derived from patient with myeloma. N.Y. State J. Med. 68(15): 2054-2060, 1968. Krueger RG et al. Tumor-associated antigens in human myeloma. J Natl Cancer Inst 56(4): 711-715, 1976. Yu S et al. IN vitro actions on human cancer cells and the liquid chromatography-mass spectrometry/mass spectrometry fingerprint of phytochemicals in rice protein isolate. J Agric Food Chem 54(12): 4482-4492, 2006. Lee KW et al. Direct role of NF-kappaB activation in Toll-like receptor-trigered HLA-DRA expression. Eur J Immunol 36(5): 1254-1266, 2006. | |
