TF-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
TF-1 |
Humane Knochenmark Erythroleukämie Zell-Linie |
300434 |
cryovial |
265,00 |
TF-1 |
Humane Knochenmark Erythroleukämie Zell-Linie |
330434 |
vital |
335,00 |
| Designation: | TF-1 |
| Depositor: | Kitamura |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Japanese |
| Age/Stage: | 35 years old |
| Gender: | male |
| Tissue: | bone marrow |
| Morphology: | lymphoblast |
| Celltype: | Erythroleukemia; erythroblast |
| Growth Properties: | Suspension |
| Description: | The TF-1 cell line has been established by T. Kitamura in October 1987 from a heparinized bone marrow aspiration sample from a 35 year old Japanese male with severe pancytopenia. They don't respond to interleukin 5 (IL-5), but to a variety of other lymphokines and cytokines such as interleukin 1 (IL-1), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 9 (IL-9), Interleukin 11 (IL-11), interleukin 13 (IL-13), stem cell factor (SCF), leukemia inhibitory factor (LIF) and nerve growth factor (NGF). The morphological and cytochemical features, and the constitutive expression of globin genes, indicate the commitment of the cells to the erythroid lineage. TPA induces a dramatic differentiation into macrophage-like cells; Hemin and delta-aminolevulinic acid induce hemoglobin synthesis. |
| Culture Medium: | RPMI 1640 medium supplemented with 1 to 5 ng/ml GM-CSF and 10% fetal bovine serum [for long term culture, TF-1 cells need interleukin 3 (IL-3, GM-CSF) in the culture medium]. |
| Subculturing: | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/ml and maintain between 1 x 105 and 1 x 106 cells/ml. |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO:13,13 D13S317: 8,9 D16S539:9,12 D5S818:13 D7S820:12 TH01:7,9 TPOX:8 vWA:15,17 D3S1358: 15 D21S11: 30 D18S51: 13 Penta E: 5,17 Penta D: 10,13 D8S1179: 11,15 FGA: 18,19 |
| Receptors Expressed: | TF-1 cells do not express glycophorin A or carbonyl anhydrase I. |
| Applications: | The TF-1 cell line can be applied in various systems due to their responsiveness to multiple cytokines. They provide a good system to investigate the proliferation and differentiation of myeloid progenitor cells. |
References: Kitamura T et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J Cell Physiol 140: 323-34, 1989. Kitamura T et al. Identification and analysis of human erythropoietin receptors on a factor-dependent cell line, TF-1. Blood 73: 375-80, 1989. Kuwaki T et al. Characterization of human interleukin-3 receptors on a multi-factor-dependent cell line. Biochem Biophys Res Commun 161: 16-22, 1989. Kitamura T et al. IL-1 up-regulates the expression of cytokine receptors on a factor- dependent human hemopoietic cell line, TF-1. Int Immunol 3: 571-7, 1991. Borellini F et al. Induction of Sp1-p53 DNA-binding heterocomplexes during granulocyte/macrophage colony-stimulating factor-dependent proliferation in human erythroleukemia cell line TF-1. J. Biol Chem 268: 7923-8, 1993. Meyn MA 3rd et al. Src family kinases phosphorylate the Bcr-Abl SH3-SH2 region and modulate Bcr-Abl transforming activity. J Biol Chem 281: 30907-16, 2006. Ehmann F et al. Detection of N-RAS and K-RAS in their active GTP-bound from in acute myeloid leukemia without activating RAS mutations. Leuk Lymphoma 47: 1387-91, 2006. | |
