THP-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
THP-1 |
Humane Monozytische Leukämie Zell-Linie |
300356 |
cryovial |
265,00 |
THP-1 |
Humane Monozytische Leukämie Zell-Linie |
330356 |
vital |
335,00 |
| Designation: | THP-1 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 1 year old |
| Gender: | male |
| Tissue: | Blood (leukemia) |
| Celltype: | Monocyte; acute monocytic |
| Growth Properties: | Suspension |
| Description: | THP-1 cells show alpha-naphtyl butyrate esterase activity, phagocytose latex particles as well as sensitized sheep erythrocytes and have the ability to restore T-lymphocyte response to Con A. When incubating with TPA or DMSO the cells can be differentiated into macrophage-like cells. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10-15% fetal bovine serum. |
| Subculturing: | Start the culture from the frozen state in centrifuging immediately to remove any traces of the freeze medium, resuspend in fresh culture medium and dispense into cell culture flasks. Subculturing into new culture flasks is recommended. Start cultures at 1 x 105 cells/ml and do not allow the cell concentration to exceed 1 x 106 cells/ml. |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,13 D13S317: 13 D16S539: 11,12 D5S818: 11,12 D7S820: 10 THO1: 8,9.3 TPOX: 8,11 vWA: 16 D3S1358: 15,17 D21S11: 30,31.2 D18S51: 13,14 Penta E: 11,15 Penta D: 10,12 D8S1179: 10,14 FGA: 24,25 CLS ∙ Cell Lines Service, 2010. |
| Immunology: | HLA haplotypes: HLA-A2, -A9, -B5, -DRw1, -DRw2 |
| Receptors Expressed: | Fc; C3b |
| Products: | Lysozyme |
References: Tsuchiya S et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer 26: 171-176, 1980. Tsuchiya S et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res 42: 1530-6, 1982. Skubitz KM et al. Human granulocyte surface molecules identified by murine monoclonal antibodies. J Immunol 131: 1882-8, 1983. Tajima S et al. Cells of a human monocytic leukemia cell line (THP-1) synthesize and secrete apolipoprotein E and lipoprotein lipase. Biochem Biophys Res Commun 126: 526-31, 1985. Huang S et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J Virol 70: 4502-8, 1996. Hambleton J et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc Natl Acad Sci USA 93: 2774-8, 1996. Hsu HY et al. Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-alpha is transcriptionally and post-transcriptionally regulated. J Biol Chem 271: 7767-73, 1996. Lucas M et al. Cell surface proteoglycans modulate net synthesis and secretion of macrophage apolipoprotein E. J Biol Chem 271: 13454-60, 1996. Ollivier V et al. Elevated cyclic AMP inhibits NF-kappaB-mediated transcription in human monocytic cells and endothelial cells. J Biol Chem 271: 20828-35, 1996. Cuthbert JA et al. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res 57: 3498-505, 1997. Clark RA et al. Tenascin supports lymphocyte rolling. J Cell Biol 137: 755-65, 1997. Ikezoe T et al. The antitumor effects of sunitinib (formerly SU11248) against a variety of human hematologic malignancies: enhancement of growth inhibition via inhibition of mammalian target of rapamycin signaling. Mol Cancer Ther 5: 2522-30, 2006. Fabian I et al. Moxifloxacin enhances antiproliferative and apoptotic effects of etoposide but inhibits its proinflammatory effects in THP-1 and Jurkat cells. Br J Cancer 95: 1038-46, 2006. | |
